Proteomics

Dataset Information

0

Lysine acetylation of F-actin decreases tropomyosin-based inhibition of actomyosin activity

ABSTRACT: Actin was acetylated on lysine residues with acetic anhydride to determine its impaction on the contractile properties of the thin filaments including, actin's ability to undergo crossbridge cycling with myosin, actin's affinity for tropomyosin, and the impact of actin acetylation on calcium regulated thin filament activation

OTHER RELATED OMICS DATASETS IN: MODEL1109130000MODEL1909260003MODEL1909260004MODEL1703310000MODEL1909260005MODEL1909260006MODEL1311110000MODEL1311110001

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Skeletal Muscle

SUBMITTER: D. Brian Foster  

LAB HEAD: Brian Foster

PROVIDER: PXD020732 | Pride | 2020-09-04

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
FileName_RO-CS-191017_CammaratoA_AC_Sample1.rawF027622.mzid.gz Mzid
FileName_RO-CS-191017_CammaratoA_AC_Sample1.rawF027622.mzid_File_Name__RO-CS-191017_CammaratoA_AC_Sample1.raw_F027622.MGF Mzid
FileName_RO-CS-191017_CammaratoA_AC_Sample1.rawF027623.mzid.gz Mzid
FileName_RO-CS-191017_CammaratoA_AC_Sample1.rawF027623.mzid_File_Name__RO-CS-191017_CammaratoA_AC_Sample1.raw_F027623.MGF Mzid
FileName_RO-CS-191017_CammaratoA_AC_Sample2.rawF027624.mzid.gz Mzid
Items per page:
1 - 5 of 11
altmetric image

Publications

Lysine acetylation of F-actin decreases tropomyosin-based inhibition of actomyosin activity.

Schmidt William W   Madan Aditi A   Foster D Brian DB   Cammarato Anthony A  

The Journal of biological chemistry 20200901 46

Recent proteomics studies of vertebrate striated muscle have identified lysine acetylation at several sites on actin. Acetylation is a reversible post-translational modification that neutralizes lysine's positive charge. Positively charged residues on actin, particularly Lys<sup>326</sup> and Lys<sup>328</sup>, are predicted to form critical electrostatic interactions with tropomyosin (Tpm) that promote its binding to filamentous (F)-actin and bias Tpm to an azimuthal location where it impedes m  ...[more]

Similar Datasets

Proteomics Identification of O-glycosylation sites in Clostridium botulinum and Geobacillus kaustophilus flagellins
2020-09-07 | PXD018167 | Pride
Proteomics Molecular basis of F-actin regulation and sarcomere assembly via myotilin
2021-02-22 | PXD024301 | Pride
Proteomics Identification of phosphorylation sites in piggyBac transposase N-terminus
2023-03-11 | PXD037132 | Pride
Proteomics Harnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras
2024-08-22 | PXD044191 | Pride
Transcriptomics RNA-seq of human fibroblast cell cultures treated with resveratrol against DMSO treated controls, with and without siRNA treatment targeting SIRT1
2018-11-12 | E-MTAB-7360 | biostudies-arrayexpress
Proteomics Simple, single-shot phosphoproteomic analysis of heat-stable tau
2021-11-25 | PXD027971 | Pride
Proteomics Depletion Assisted Hemin Affinity (DAsHA) Proteomics Reveals an Expanded Landscape of Heme Binding Proteins in the Human Proteome
2023-05-10 | PXD037165 | Pride
Genomics Local DNA hypomethylation activates genes in rice endosperm
2010-11-01 | E-GEOD-22591 | biostudies-arrayexpress
Proteomics Capacitive electrical stimulation effects on osteoblast-released matrix microvesicles
2021-11-29 | PXD028551 | Pride
Proteomics Integrated phosphoproteome, ubiquitinome, and acetylome analyses uncovering the post-translational modifications crosstalk during MSP1-induced signaling in rice
2023-07-21 | PXD039997 | Pride