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Cellular proteins interacting with curved HIV-1 capsid lattices

ABSTRACT: Early events of HIV-1 lifecycle, such as post-entry virus trafficking, uncoating and nuclear import, remain poorly understood due to limited information about virus-host interactions. We used a multidisciplinary approach. First, a mass spectrometry (MS)-based proteomics methodology enabled us to identify cellular proteins that specifically bind to 3-dimensional curved hexameric capsid lattices closely mimicking the assembled HIV-1 core. We then employed virology, live cell imaging, CRISPR gene knockout, x-ray crystallography, and biochemistry to demonstrate that Sec24C (a component of the COPII complex) is the first example of a cytoplasmic protein that utilizes an FG containing motif to directly and specifically bind the curved hexameric HIV-1 capsid lattices.

INSTRUMENT(S): LTQ Orbitrap XL, Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

SUBMITTER: Nikoloz Shkriabai

LAB HEAD: Mamuka Kvaratskhelia

PROVIDER: PXD020970 | Pride | 2021-01-04

REPOSITORIES: Pride

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			Action	DRS
	F115965.mgf	Mgf
	F116455.mgf	Mgf
	F116456.mgf	Mgf
	F116458.mgf	Mgf
	F116459.mgf	Mgf

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