Proteomics

Dataset Information

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RBPome of primary human T helper cells


ABSTRACT: Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about the entire RBPome involved in post-transcriptional gene regulation in T cells is lacking. Here, we applied global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS) to human and mouse primary T cells and identified the core T cell RBPome. This defined 798 mouse and 801 human proteins as RBPs, unexpectedly containing signaling proteins like Stat1, Stat4 and Vav1. The data represent an atlas of RNA-binding proteins in human and mouse T helper cells as a resource for studying higher order post-transcriptional gene regulation.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Cell Culture

SUBMITTER: Michael Wierer  

LAB HEAD: Michael Wierer

PROVIDER: PXD021164 | Pride | 2021-07-01

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Human_RNAIC_Th0_CL_1.raw Raw
Human_RNAIC_Th0_CL_2.raw Raw
Human_RNAIC_Th0_CL_3.raw Raw
Human_RNAIC_Th0_nCL_1.raw Raw
Human_RNAIC_Th0_nCL_2.raw Raw
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