Dataset Information

Saw-scaled viper, Echis carinatus sochureki, venomics

ABSTRACT: we used a combination of bottom-up (BU) and top-down (TD) proteomics approaches to identify and characterize venom protein compositions of Echis carinatus sochureki (ECS) from three different Iranian populations.

INSTRUMENT(S): Q Exactive HF-X, Q Exactive

ORGANISM(S): Echis Carinatus Sochureki

TISSUE(S): Venom

SUBMITTER: Parviz Ghezellou

LAB HEAD: Bernhard Spengler

PROVIDER: PXD021183 | Pride | 2021-01-29

REPOSITORIES: Pride

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Publications

Integrating Top-Down and Bottom-Up Mass Spectrometric Strategies for Proteomic Profiling of Iranian Saw-Scaled Viper, Echis carinatus sochureki, Venom.

Ghezellou Parviz P Albuquerque Wendell W Garikapati Vannuruswamy V Casewell Nicholas R NR Kazemi Seyed Mahdi SM Ghassempour Alireza A Spengler Bernhard B

Journal of proteome research 20201122 1

Saw-scaled or carpet vipers (genus Echis) are considered to cause a higher global snakebite mortality than any other snake. Echis carinatus sochureki (ECS) is a widely distributed snake species, also found across the thirteen provinces of Iran, where it is assumed to be responsible for the most snakebite envenomings. Here, we collected the Iranian specimens of ECS from three different geographically distinct populations, investigated food habits, and performed toxicity assessment a ...[more]

PMID: 33225711

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Project description:GOAL: Identification of genes and/or pathways involved in the pathogenesis of animal models of Rheumatoid Arthritis (RA). Total RNA samples were extracted from synovial fibroblast ex vivo cultures or whole joints (J), isolated from arthritic mice (197, DARE) and their corresponding normal (F1, wt) littermates. Fluorescent-labeled cRNA probes were utilized to hybridize (in duplicates for 197, indicated by "new") the Affymetrix Mu11K oligonucleotide DNA chipset (A+B). In detail the samples are: <table> <tr> <td> Animal model </td> <td> Sample source </td> <td> Sample name </td> </tr> <tr> <td> Tg197 </td> <td> Tg197 ex-vivo cultured synoviocyte populations </td> <td> 197 </td> </tr> <tr> <td> Tg197 </td> <td> Tg197 ex-vivo cultured synoviocyte populations </td> <td> 197new </td> </tr> <tr> <td> Tg197 </td> <td> wt ex-vivo cultured synoviocyte populations </td> <td> F1 </td> </tr> <tr> <td> Tg197 </td> <td> wt ex-vivo cultured synoviocyte populations </td> <td> F1new </td> </tr> <tr> </tr> <tr> <td> Tg197 </td> <td> Tg197 whole joints </td> <td> 197J </td> </tr> <tr> <td> Tg197 </td> <td> Tg197 whole joints </td> <td> 197Jnew </td> </tr> <tr> <td> Tg197 </td> <td> wt whole joints </td> <td> F1J </td> </tr> <tr> <td> Tg197 </td> <td> wt whole joints </td> <td> F1Jnew </td> </tr> <tr> </tr> <tr> <td> DARE </td> <td> DARE ex-vivo cultured synoviocyte populations </td> <td> DARE </td> </tr> <tr> <td> DARE </td> <td> wt ex-vivo cultured synoviocyte populations </td> <td> wt </td> </tr> <tr> <td> DARE </td> <td> DARE whole joints </td> <td> DAREJ </td> </tr> <tr> <td> DARE </td> <td> wt whole joints </td> <td> wtJ </td> </tr> </table> Submitted files carry the extension A or B, corresponding to the subArrays of chipset Mu11K. Arthritic samples were always compared with the corresponding wt sample and not to a common reference chip.

			Action	DRS
	Echismix_1_TD_NR.raw	Raw
	Echismix_1_TD_R.raw	Raw
	Echismix_2_TD_NR.raw	Raw
	Echismix_2_TD_R.raw	Raw
	Echismix_3_TD_NR.raw	Raw

Dataset Information

Saw-scaled viper, Echis carinatus sochureki, venomics

Dataset's files

Publications

Integrating Top-Down and Bottom-Up Mass Spectrometric Strategies for Proteomic Profiling of Iranian Saw-Scaled Viper, <i>Echis carinatus sochureki</i>, Venom.

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