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Profiling extra cellular matrix core proteome of human fetal nucleus pulposus in search for regenerative targets

ABSTRACT: Intervertebral disc degeneration is accompanied by a loss of Extra-cellular matrix (ECM) content due to an imbalance in anabolic and catabolic pathways. Identifying ECM proteins with anabolic and/or regenerative potential could be the key to developing regenerative therapies. Since human fetal discs grow and develop very rapidly, studying these discs may provide valuable insights on proteins with regenerative potential. This study compares core matrisome of 9 fetal and 7 healthy adult (age 22-79) nucleus pulposus (NP), using a proteomic and bioinformatic approach. In order to assess whether the protein that decrease from fetus to healthy adult NP’s further decrease in severely degenerated discs, expression levels of all proteins of interest that were identified in regenerative pathways were used for an additional analysis

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Chondrocyte, Nucleus Pulposus, Lumbar Spine, Notochordal Cell, Fibroblast

DISEASE(S): Degenerative Disc Disease

SUBMITTER: Chitraa Tangavel

LAB HEAD: Dr Chitraa Tangavel

PROVIDER: PXD021431 | Pride | 2025-01-07

REPOSITORIES: Pride

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			Action	DRS
	DD10_NP_S1_Pool1_1.mgf	Mgf
	DD10_NP_S1_Pool1_1.mzML	Mzml
	DD10_NP_S1_Pool1_1.mzid.gz	Mzid
	DD10_NP_S1_Pool1_1.raw	Raw
	DD10_NP_S1_Pool1_2.mgf	Mgf

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