Proteomics

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Proteomic Signature of Nucleus Pulposus of Human Intervertebral Disc

ABSTRACT: Intervertebral Disc (IVD) degeneration leading to Low back pain (LBP) is the most common musculoskeletal disorder. Lack of knowledge on the intricate homeostatic mechanisms necessitates proteomic characterisation of a normal human IVD to understand the biological process and unravel the pathomechanisms of degenerative disc disease (DDD). In this study, we employed proteomic approach coupled with tandem mass-spectrometry to derive the comprehensive list of proteins expressed in true biologically normal control discs. This would serve as the basis for identifying the interacting molecules participating in significant biological processes and pathways disrupted during aging and degeneration.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Chondrocyte, Nucleus Pulposus

DISEASE(S): Degenerative Disc Disease

SUBMITTER: Chitraa Tangavel  

LAB HEAD: Chitraa Tangavel

PROVIDER: PXD016560 | Pride | 2021-09-09

REPOSITORIES: Pride

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Action DRS
CT_T058_NP_S1_Pool1_1.mgf Mgf
CT_T058_NP_S1_Pool1_1.mzML Mzml
CT_T058_NP_S1_Pool1_1.mzid.gz Mzid
CT_T058_NP_S1_Pool1_1.pride.mgf.gz Mgf
CT_T058_NP_S1_Pool1_1.raw Raw
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Publications

Proteomic Signatures of Healthy Intervertebral Discs From Organ Donors: A Comparison With Previous Studies on Discs From Scoliosis, Animals, and Trauma.

Rajasekaran Shanmuganathan S   Tangavel Chitraa C   Soundararajan Dilip Chand Raja DCR   Nayagam Sharon Miracle SM   Matchado Monica Steffi MS   Muthurajan Raveendran R   Anand K S Sri Vijay KSSV   Rajendran Sunmathi S   Shetty Ajoy Prasad AP   Kanna Rishi Mugesh RM   Kuppamuthu Dharmalingam D  

Neurospine 20200630 2

<h4>Objective</h4>To catalog and characterize the proteome of normal human intervertebral disc (IVD).<h4>Methods</h4>Nine magnetic resonance imaging (MRI) normal IVDs were harvested from 9 different brain dead yet alive voluntary organ donors and were subjected to electrospray ionization-liquid chromatography tandem mass spectrometry (ESI-LC-MS/MS) acquisition.<h4>Results</h4>A total of 1,116 proteins were identified. Functional enrichment analysis tool DAVID ver. 6.8 categorized: extracellular  ...[more]

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