Proteomics

Dataset Information

0

Stearic acid blunts growth-factor signaling via oleoylation of GNAI proteins


ABSTRACT: The aim of the MS analysis is to identify fatty acids modifying Cys3 of GNAI3. Detailed description in the manuscript Nuskova et al. submitted to NatComms.

INSTRUMENT(S): ultraflex

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Hana Nuskova  

LAB HEAD: Aurelio A. Teleman

PROVIDER: PXD021517 | Pride | 2021-07-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
181121AnalysisParameter.xml Xml
181121GNAI3endogenousacylation.zip Other
181121MascothomeGNAI3-GFPms1.mht Other
181121MascothomeGNAI3-GFPms2.mht Other
19030113C18_0.zip Other
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Publications


Covalent attachment of C16:0 to proteins (palmitoylation) regulates protein function. Proteins are also S-acylated by other fatty acids including C18:0. Whether protein acylation with different fatty acids has different functional outcomes is not well studied. We show here that C18:0 (stearate) and C18:1 (oleate) compete with C16:0 to S-acylate Cys3 of GNAI proteins. C18:0 becomes desaturated so that C18:0 and C18:1 both cause S-oleoylation of GNAI. Exposure of cells to C16:0 or C18:0 shifts GNA  ...[more]

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