Dataset Information

Comparing Endocervical Mucus Proteome of Humans and Rhesus Macaques

ABSTRACT: Endocervical mucus changes play a key role in regulating fertility throughout the menstrual cycle and in response to hormonal contraceptives. Non-human primates (NHP) provide the most translational animal model for reproductive tract studies, as they have hormonally-regulated menstrual cycles and mucus changes, similar to women. We used TMT labelling and LC-LC/MS to compare the proteins found in the mucus of the rhesus macaque to the mucus of the human endocervix. We found 3,048 total proteins present in both rhesus mucus and human mucus, and of these, 57% showed a similar expression pattern. An even higher similarity occurred in the top 500 most prevalent proteins, with overlap in 341 (68%) proteins. Mucin MUC5B was the most highly expressed mucin protein (top 10 expressed proteins in both) but other key proteins related to mucus structure were present in both samples. We find that the mucus proteome of the endocervical mucus is highly conserved in NHP and women. This supports use of the NHP model system for studies of the endocervix and trials of novel fertility treatments targeting the cervix.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human) Macaca Mulatta (rhesus Macaque)

TISSUE(S): Cervical Mucosa

SUBMITTER: Phillip Wilmarth

LAB HEAD: Dr. Leo Han

PROVIDER: PXD021710 | Pride | 2021-05-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2019.06_UP000005640_9606_Homo_sapiens_canonical_both.fasta	Fasta
	2019.07_UP000006718_9544_Macaca_mulatta_canonical_both.fasta	Fasta
	HAN-743_Data_Analysis.xlsx	Xlsx
	HAN-743_MacaquePrimaryCells_Macaque_Human_Mucus_TMT_2DRPRP_135ug_25cm_120min_14ACN_20190816_OT.ms2.gz	Other
	HAN-743_MacaquePrimaryCells_Macaque_Human_Mucus_TMT_2DRPRP_135ug_25cm_120min_14ACN_20190816_OT.raw	Raw

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Publications

Comparing endocervical mucus proteome of humans and rhesus macaques.

Han Leo L Park Daye D Reddy Ashok A Wilmarth Phillip A PA Jensen Jeffrey T JT

Proteomics. Clinical applications 20210526 4

<h4>Purpose</h4>Endocervical mucus changes play a key role in regulating fertility throughout the menstrual cycle and in response to hormonal contraceptives. Non-human primates (NHP) provide the most translational animal model for reproductive tract studies, as they have hormonally-regulated menstrual cycles and mucus changes, similar to women.<h4>Experimental design</h4>We used TMT labelling and LC-LC/MS to compare the proteins found in the mucus of the rhesus macaque to the mucus of the human ...[more]

PMID: 33991406

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