Dataset Information

Inhibition of protein N-myristoylation blocks Plasmodium falciparum intracellular development and egress, but myristoylation of GAP45 is required only for erythrocyte invasion.

ABSTRACT: Inhibition of protein N-myristoylation blocks Plasmodium falciparum intracellular development and egress, but myristoylation of GAP45 is required only for erythrocyte invasion. TMT quantification used to determine protein expression changes upon inhibition of myristoylation

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

DISEASE(S): Plasmodium Falciparum Malaria

SUBMITTER: Steven Howell

LAB HEAD: Bram Snijders

PROVIDER: PXD021879 | Pride | 2021-10-07

REPOSITORIES: Pride

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			Action	DRS
	PAH9031A1RW2_A1PAH9031A1RW2-40-555095.raw	Raw
	PAH9031A2RW2_A2PAH9031A2RW2-40-555096.raw	Raw
	PAH9031A3RW2_A3PAH9031A3RW2-40-555097.raw	Raw
	PAH9031A4RW2_A4PAH9031A4RW2-40-555098.raw	Raw
	PAH9031A5RW2_A5PAH9031A5RW2-40-555099.raw	Raw

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Project description:Polyamines are aliphatic polycations that have emerged as important determinants of cell growth and viability in rapidly proliferating cells, including in the pathogenic protozoan parasite Leishmania donovani. In L. donovani, the polyamine spermidine is synthesized by the successive conversion of ornithine into putrescine (catalyzed by ornithine decarboxylase or ODC) and putrescine into spermidine (catalyzed by spermidine synthase or SPDSYN). Deletion of either ODC (del-odc) or SPDSYN (del-spdsyn) from the L. donovani genome renders these parasites auxotrophic for polyamines and these mutants are impaired in their ability to survive both in culture and within the mammalian host without the addition of exogenous polyamine supplementation. Significantly, del-odc parasites immediately cease proliferation after putrescine is removed from the culture media and perish within two weeks, while spermidine starved del-spdsyn mutants, which retain intracellular putrescine pools, show a slow-growth phenotype, and persist for several weeks in culture. To elucidate the key differences within the proteome of putrescine-starved del-odc cells and spermidine-starved del-spdsyn parasites, a shotgun quantitative proteomics approach was undertaken using TMT labeling and LC-MS/MS analysis. Briefly, three biological replicates each for mid-log phase del-odc and del-spdsyn promastigotes grown in the presence of exogenous putrescine (for del-odc) or spermidine (for del-spdsyn) supplementation were washed to remove the exogenous polyamine supplementation and incubated in polyamine-free media. At 24 and 48 h, cells from each biological replicate were isolated and prepared for tandem mass tag (TMT) labeling and downstream LC-MS/MS analyses. Peptides were identified using a database generated from the reference genome of L. donovani BPK282A1 strain. Changes in relative protein abundance for the polyamine-starved del-odc and del-spdsyn cell lines at 24 and 48 h were calculated by comparing aggregate total reporter ion intensities for each protein to that of the corresponding polyamine-supplemented 0-h timepoint.

Dataset Information

Inhibition of protein N-myristoylation blocks Plasmodium falciparum intracellular development and egress, but myristoylation of GAP45 is required only for erythrocyte invasion.

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