Dataset Information

Root shotgun proteomics from Fe- and Mn-deficient and Mn-toxic tomato plants

ABSTRACT: The aim of this work was to study the effects of Fe and Mn deficiencies and Mn toxicity on the protein profile of the root of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published. The high-throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato root. Protein identification was carried out using the Mascot search engine and the non-redundant databases NCBInr and ITAG v2.3.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Plant Cell, Root

SUBMITTER: Laura Ceballos-Laita

LAB HEAD: Laura Ceballos-Laita

PROVIDER: PXD008326 | Pride | 2018-08-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ITAG2.3_database.fasta.txt	Fasta
	Mascotpeptideinformation_ITAGdatabase.csv	Csv
	Mascotpeptideinformation_NCBI.csv	Csv
	Mascotproteininformation_ITAGdatabase.csv	Csv
	Mascotproteininformation_NCBI.csv	Csv

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Publications

Effects of manganese toxicity on the protein profile of tomato (Solanum lycopersicum) roots as revealed by two complementary proteomic approaches, two-dimensional electrophoresis and shotgun analysis.

Ceballos-Laita Laura L Gutierrez-Carbonell Elain E Imai Hiroyuki H Abadía Anunciación A Uemura Matsuo M Abadía Javier J López-Millán Ana Flor AF

Journal of proteomics 20180625

The aim of this work was to assess the effects of manganese (Mn) toxicity on the proteome of tomato roots using two proteomic approaches, shotgun and two-dimensional electrophoresis. The shotgun approach yielded 367 reliable proteins, whereas the 2-DE approach detected 340 consistent spots. The 2-DE method found 54 proteins changing in relative abundance in the excess Mn treatment, whereas the shotgun detected changes in 118 proteins. Only 7% of the differential proteins were found by both metho ...[more]

PMID: 29953959

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