Proteomics

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Exploring the interaction between the SWI/SNF chromatin remodeling complex and the zinc finger factor CTCF


ABSTRACT: The transcription factor CCCTC-binding factor (CTCF) modulates pleiotropic functions mostly related to gene expression regulation. The role of CTCF in large scale genome organization is also well established. A unifying model to explain relationships between many CTCF-mediated activities involve direct or indirect interactions with numerous protein cofactors recruited to specific binding sites. The co-association of CTCF with other architectural proteins such as cohesin, chromodomain helicases and BRG1 further support the interplay between master regulators of mammalian genome folding. Here we report a comprehensive LC-MS/MS mapping of the components of the SWI/SNF chromatin remodeling complex co-associated with CTCF including subunits belonging to the core, signature and ATPase modules. We further show that the localization patterns of representative SWI/SNF members significantly overlap with CTCF sites on transcriptionally active chromatin regions. Moreover, we provide evidence of a direct binding of the BRK-BRG1 domain to the zinc finger motifs 4-8 of CTCF, thus suggesting that these domains mediate the interaction of CTCF with the SWI/SNF complex. These findings provide an updated view of the cooperative nature between CTCF and the SWI/SNF ATP-dependent chromatin-remodeling complexes, an important step for understanding how these architectural proteins collaborate to shape the genome.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Angela Chambery  

LAB HEAD: Angela Chambery

PROVIDER: PXD022037 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CTCF_IPBenzonase_2_Homo.msf Msf
CTCF_IPBenzonase_2_SWI_SNF.msf Msf
CTCF_IPBenzonase_3_Homo.msf Msf
CTCF_IPBenzonase_3_SWI_SNF.msf Msf
CTCF_IP_Benzonase.raw Raw
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Publications

Exploring the Interaction between the SWI/SNF Chromatin Remodeling Complex and the Zinc Finger Factor CTCF.

Valletta Mariangela M   Russo Rosita R   Baglivo Ilaria I   Russo Veronica V   Ragucci Sara S   Sandomenico Annamaria A   Iaccarino Emanuela E   Ruvo Menotti M   De Feis Italia I   Angelini Claudia C   Iachettini Sara S   Biroccio Annamaria A   Pedone Paolo Vincenzo PV   Chambery Angela A  

International journal of molecular sciences 20201125 23


The transcription factor CCCTC-binding factor (CTCF) modulates pleiotropic functions mostly related to gene expression regulation. The role of CTCF in large scale genome organization is also well established. A unifying model to explain relationships among many CTCF-mediated activities involves direct or indirect interactions with numerous protein cofactors recruited to specific binding sites. The co-association of CTCF with other architectural proteins such as cohesin, chromodomain helicases, a  ...[more]

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