Proteomics

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Medieval birth girdle stains LC-MSMS


ABSTRACT: This project applied a dry non-invasive method to detect palaeoproteomic evidence from stained manuscripts. The manuscript analysed in this study is a medieval parchment birth girdle (Wellcome Collection Western MS. 632) made in England and thought to be used by pregnant women while giving birth. Using a dry non-invasive sampling method we were able to extract both human and non-human peptides from the stains, including evidence for the use of honey, cereals, ovicaprine milk and legumes. In addition, a large number of human peptides were detected on the birth roll, many of which are found in cervico-vaginal fluid. This suggests that the birth roll was actively used during childbirth. This study is the first to extract and analyse non-collagenous peptides from a parchment document using a dry non-invasive sampling method and demonstrates the potential of this type of analysis for stained manuscripts, providing direct biomolecular evidence for active use.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human) Ovis Aries Pisum Sativum (garden Pea) Vicia Faba Apis Mellifera (honeybee)

SUBMITTER: Sarah Fiddyment  

LAB HEAD: Matthew James Collins

PROVIDER: PXD022054 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Girding the loins? Direct evidence of the use of a medieval English parchment birthing girdle from biomolecular analysis.

Fiddyment Sarah S   Goodison Natalie J NJ   Brenner Elma E   Signorello Stefania S   Price Kierri K   Collins Matthew J MJ  

Royal Society open science 20210310 3


In this paper, we describe palaeoproteomic evidence obtained from a stained medieval birth girdle using a previously developed dry non-invasive sampling technique. The parchment birth girdle studied (Wellcome Collection Western MS. 632) was made in England in the late fifteenth century and was thought to be used by pregnant women while giving birth. We were able to extract both human and non-human peptides from the manuscript, including evidence for the use of honey, cereals, ovicaprine milk and  ...[more]

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