Proteomics

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ULK1 regulated phosphosites in mouse and human cells

ABSTRACT: (1)In vivo SILAC analyses of mouse embryonic fibroblasts (MEFs, triple labeling) were performed comparing phosphorylation events. ULK1 double knock out MEFs (VC) were compared to double knock out MEFs expressing human ULK1 (RE) in fed (DMEM) and starvation (HBSS) conditions. (2) In vivo SILAC analyses of A549 cells (triple labeling) were performed comparing phosphorylation events (195 raw files labeled ""A549""). A549 cells in fed conditions (DMEM) were compared to starved cells (HBSS) and cells treated with rapamycin (Rapa).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Fibroblast

SUBMITTER: Joern Dengjel  

LAB HEAD: Joern Dengjel

PROVIDER: PXD022303 | Pride | 2021-11-03

REPOSITORIES: Pride

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20190607_ZH_A549_Rapa_WT_STA_FT_1_01.raw Raw
20190607_ZH_A549_Rapa_WT_STA_FT_1_01_20190608154212.raw Raw
20190607_ZH_A549_Rapa_WT_STA_FT_1_02.raw Raw
20190607_ZH_A549_Rapa_WT_STA_FT_1_02_20190608174718.raw Raw
20190607_ZH_A549_Rapa_WT_STA_FT_1_03.raw Raw
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Publications

The evolutionarily conserved ULK1 kinase complex acts as gatekeeper of canonical autophagy and regulates induction of autophagosome biogenesis. To better understand control of ULK1 and analyze whether ULK1 has broader functions that are also linked to the later steps of autophagy, we perform comprehensive phosphoproteomic analyses. Combining in vivo with in vitro data, we identify numerous direct ULK1 target sites within autophagy-relevant proteins that are critical for autophagosome maturation  ...[more]

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