Dataset Information

WDR4 conditional knockout in mouse cerebellar granule progenitors for proteome analysis

ABSTRACT: To uncover candidates which are WDR4 downstream effectors regulating cerebellar granule neuron proliferation phenotype, we utilized quantitative proteomics analysis (TMT labeling) with granule neuron progenitors from P7 wdr4 conditional knockout (using Atoh1-Cre) and control cerebella.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Cerebellar Granule Cell

SUBMITTER: Pei-Rung Wu

LAB HEAD: Ruey-Hwa Chen

PROVIDER: PXD022319 | Pride | 2023-03-11

REPOSITORIES: Pride

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Publications

Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation.

Wu Pei-Rung PR Chiang Shang-Yin SY Midence Robert R Kao Wen-Chao WC Lai Chun-Lun CL Cheng I-Cheng IC Chou Shen-Ju SJ Chen Chih-Cheng CC Huang Chih-Yang CY Chen Ruey-Hwa RH

Cell death & disease 20230121 1

Patients with mutations of WDR4, a substrate adaptor of the CUL4 E3 ligase complex, develop cerebellar atrophy and gait phenotypes. However, the underlying mechanisms remain unexplored. Here, we identify a crucial role of Wdr4 in cerebellar development. Wdr4 deficiency in granule neuron progenitors (GNPs) not only reduces foliation and the sizes of external and internal granular layers but also compromises Purkinje neuron organization and the size of the molecular layer, leading to locomotion de ...[more]

PMID: 36681682

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	cm_190322.mgf	Mgf
	cm_190322.raw	Raw
	cm_f1_190322.mgf	Mgf
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	cm_f2_190322.mgf	Mgf