Proteome and phosphoproteome of human bladder cancer tissue
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF-X, Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Bladder Cell, Bladder
DISEASE(S): Urinary Bladder Cancer
SUBMITTER: 
Valentin SABATET
LAB HEAD: Damarys Loew
PROVIDER: PXD022435 | Pride | 2022-05-12
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| C6945FD.msf | Msf | |||
| C6945FD.raw | Raw | |||
| C6946FD.msf | Msf | |||
| C6946FD.raw | Raw | |||
| C6947FD.msf | Msf |
Items per page: 5 1 - 5 of 1241 |
Similar Datasets
Project description:The identification of miRNAs’ targets and associated regulatory networks might allow the definition of new strategies using drugs whose association might mimic a given miRNA’s effects. Based on this assumption our group devised a multi-omics approach in an attempt to precisely characterize miRNAs’ effects. We combined the analysis of miR-491-5p direct targets, and effects at the transcriptomic and proteomic levels. We thus constructed an interaction network which enlightened highly connected nodes, being either direct or indirect targets of miR-491-5p effects: the already known EGFR and BCL2L1, but also EP300, CTNNB1 and several small-GTPases. By using different combinations of specific inhibitors of these nodes, we could greatly enhance their respective cytotoxicity and mimic miR-491-5p-induced phenotype. Our methodology thus constitutes an interesting strategy to comprehensively study the effects of a given miRNA. Also, we identified targets for which pharmacological inhibitors are already available for a clinical use, or in clinical trial phases. This study might thus enable innovative therapeutic options for ovarian cancer, which remains the first cause of death from gynecological malignancies in developed countries.
2021-09-09 | PXD022131 | Pride
Project description:To explore the role of microglial TNFα in the control of sleep through phosphorylation. Specifically, to examine the involvement of microglial TNFα in the control of brain phosphorylation along the sleep-wake cycle and in the phosphorylation-based coding of sleep need.
2022-11-07 | PXD030568 | Pride
Project description:In order to understand which molecular mechanisms are involved in radiation resistance and tumor propagation of glioblastoma, our laboratory has developed in-vitro models capable of mimicking the perivascular niche. Indeed, our laboratory hypothesizes that this niche is essential for glioblastoma cells to acquire a radioresistant character. That is why we are considering culturing our glioblastoma cells (GL261 cells) with the factors secreted by the perivascular niche (conditioned medium of mouse brain blood vessels) in order to study which phosphoproteins are activated in the presence of these factors. In parallel, a RNA sequencing analysis was carried out
2024-01-23 | PXD042606 | Pride
Project description:We set up a zebrafish model to study EVs in vivo by light microscopy, using CD63-pHluorin as a marker. To verify if exosomes from zebrafish have a comparable composition as human exosomes, and to validate the use of CD63 as a marker, we first analysed the compositon of EVs isolated from in vitro cultured fibroblasts (AB.9 ATCC), and could detect the presence of conventional exosome markers, including (zebrafish) CD63. In live embryos, we could track a population of exosomes from their source (the yolk syncytial layer (YSL)) to their final destination by live microscopy. To assess the composition of these exosomes, we dissociated YSL:CD63-pHluorin expressing 3dpf embryos and isolated exosomes from the supernatant and enriched for EVs from the YSL. This was compared to a background of total EVs isolated the control fish (non-expressing).
2019-04-10 | PXD011258 | Pride
Project description:Stat3 has been acknowledged as an oncogene that has dual protumorigenic activity in a cell-intrinsic way, by promoting cancer cell proliferation and survival and in a paracrine mode, acting as suppressor of immune cell attack. We demonstrated that Stat3 silencing with a small interfering RNA (siRNA) induced a senescence program in Stat3-addicted cancer cells and leads to the production of a senescence associated secretory phenotype (SASP) that has several antitumor properties. The composition of the SASP induced by Stat3 silencing is poorly characterized. The goal of the project was to analyze the protein content in the secretome released by the mouse breast cancer cell line 4T1 after Stat3 silencing with a small interfering RNA.
2020-01-21 | PXD013948 | Pride
Project description:Cdc42 is a small GTPase protein whose role in polarity is widely studied. However, in vertebrates there exist 2 isoforms arising due to alternative splicing. The function and localization of these have not been studied with respect to cell polarity and cell migration. We therefore perform a mass spec screen to identify interactors of the isoforms to better understand their cellular functions.
2024-01-23 | PXD017477 | Pride
Project description:Characterisation of the extracellular vesicles shed by follicular lymphoma B cells that induce the polarization of bone marrow stroma cell toward a pro-tumoral niche.
2021-02-08 | PXD023749 | Pride
Project description:This study focuses on the impact of ECM (extra-cellular matrix) over cell processes such as proliferation, differentiation or mineralization which is more specifically related to our dental pulp cells. We cultured these dental pulp stem cells (DPSC) in mineralization growth or normal growth conditions. After 21 days, cells were incubated in decellularized solution until no intact cells are seen. After ECM was washed, we studied the mineralization associated to these two different ECM. Matrisome proteins were identified through proteomics. DPSC matrisome is composed of 225 individual different proteins. We classified them according to different categories, the 3 core matrisome categories: glycoproteins, collagens, proteoglycans and the 3 matrisome associated proteins categories: the regulators, affiliated and secreted. When comparing the proteins in the N-ECM and OM-ECM, most of the core matrisome proteins are downregulated in OM conditions, except 3 glycoproteins, as well as regulators and secreted factors. However, annexins were found to be upregulated in OM condition. Cell adhesion, tensile strength and growth factor binding are over-represented in NM ECM. The collagen group and glycoproteins were higher in N-ECM than OM-ECM Thereafter, gingival stem cells (GSC), the less inherit osteogenic potency cells, were seeded on these N-ECM and OM-ECM. When GSCs were seeded on DPSC-derived ECM, the OM-ECM dramatically promoted mineral deposition compared with N-ECM. We hypothesize that annexins could participate in the osteogenic inductive properties. ECM plays a pivotal role in many physiological processes, it regulates cells behavior and can orient cell differentiation. Dental pulp and oral mucosa share embryological origins but differ in their reactions to insults. Dental pulp can mineralize while oral mucosa heals ad integrum. We hypothesize that ECM participate in these characteristics.
2021-09-23 | PXD018951 | Pride
Project description:Streptococcus gallolyticus subsp. gallolyticus (SGG), an opportunistic gram-positive pathogen responsible for septicemia and endocarditis in the elderly, is often associated with colon cancer (CRC). In this work, we investigated the oncogenic role of SGG strain UCN34 using the azoxymethane (AOM)-induced CRC model in vivo, organoids formation ex vivo and full proteomic and phosphoproteomic analysis from murine colons. To identify SGG-specific pathogenic traits, the choice of the control bacterium was important, and we selected the genetically closest and non-pathogenic relative of SGG named S. gallolyticus subsp. macedonicus (SGM). We showed that SGG UCN34 accelerates colon tumor development in the murine CRC model. To test SGG’s capacity to induce pre-cancerous transformation of the murine colonic epithelium, we grew ex vivo organoids which revealed unusual structures with compact morphology. To understand the molecular changes induced by SGG UCN34, we compared full proteome and phosphoproteome analysis of murine colon chronically colonized by SGG UCN34 or SGM. We found that 136 proteins and 583 phosphorylation sites were differentially regulated following colonization by SGG UCN34. Ingenuity Pathway Analysis (IPA) indicates a pro-tumoral shift induced specifically with SGG UCN34, as most proteins and phosphoproteins identified were associated with digestive cancer. Comprehensive analysis of the altered phosphoproteins using ROMA software revealed possible activation by SGG UCN34 of several cancer hallmark pathways, i.e. MAPK (ERK, JNK and p38), mTOR and integrin/ILK/actin signaling. Altogether, our results reveal for the first time that the oncogenic role of SGG UCN34 is associated with activation of multiple cancer-related signaling pathways which cannot be recapitulated in basic in vitro culture models.
2023-09-22 | PXD038272 | Pride
Project description:Streptococcus gallolyticus subsp. gallolyticus (SGG), an opportunistic gram-positive pathogen responsible for septicemia and endocarditis in the elderly, is often associated with colon cancer (CRC). In this work, we investigated the oncogenic role of SGG strain UCN34 using the azoxymethane (AOM)-induced CRC model in vivo, organoids formation ex vivo and full proteomic and phosphoproteomic analysis from murine colons. To identify SGG-specific pathogenic traits, the choice of the control bacterium was important, and we selected the genetically closest and non-pathogenic relative of SGG named S. gallolyticus subsp. macedonicus (SGM). We showed that SGG UCN34 accelerates colon tumor development in the murine CRC model. To test SGG’s capacity to induce pre-cancerous transformation of the murine colonic epithelium, we grew ex vivo organoids which revealed unusual structures with compact morphology. To understand the molecular changes induced by SGG UCN34, we compared full proteome and phosphoproteome analysis of murine colon chronically colonized by SGG UCN34 or SGM. We found that 136 proteins and 583 phosphorylation sites were differentially regulated following colonization by SGG UCN34. Ingenuity Pathway Analysis (IPA) indicates a pro-tumoral shift induced specifically with SGG UCN34, as most proteins and phosphoproteins identified were associated with digestive cancer. Comprehensive analysis of the altered phosphoproteins using ROMA software revealed possible activation by SGG UCN34 of several cancer hallmark pathways, i.e. MAPK (ERK, JNK and p38), mTOR and integrin/ILK/actin signaling. Altogether, our results reveal for the first time that the oncogenic role of SGG UCN34 is associated with activation of multiple cancer-related signaling pathways which cannot be recapitulated in basic in vitro culture models.
2023-09-22 | PXD038268 | Pride