Proteomics

Dataset Information

0

The PI3Kδ interactome in naïve and activated mouse CD4+ T cells

ABSTRACT: Phosphoinositide 3-kinases (PI3Ks) play a central role in adaptive immunity by transducing signals from the T cell antigen receptor (TCR) via production of PIP3. PI3Kδ is a heterodimer composed of a p110δ catalytic subunit associated with a p85α or p85β regulatory subunit and is preferentially engaged by the TCR upon T cell activation. The molecular mechanisms leading to PI3Kδ recruitment and activation at the TCR signalosome remain unclear. In this study, we have used affinity purification coupled with quantitative mass spectrometry to uncover the p110δ interactome in primary CD4+ T cells. Moreover, we have determined how the PI3Kδ interactome changes upon the differentiation of small naïve T cells into T cell blasts expanded in the presence of IL-2.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cd4 Positive T Lymphocyte, Primary Cell, T Cell

SUBMITTER: Daisy Luff  

LAB HEAD: Klaus Okkenhaug

PROVIDER: PXD022607 | Pride | 2021-03-08

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Ref_717-16_50pc_1700V.raw Raw
Ref_717-17_50pc_1700V.raw Raw
Ref_717-18_50pc_1700V.raw Raw
Ref_717-19_50pc.raw Raw
Ref_717-20_50pc.raw Raw
Items per page:
1 - 5 of 54
altmetric image

Publications

PI3Kδ Forms Distinct Multiprotein Complexes at the TCR Signalosome in Naïve and Differentiated CD4<sup>+</sup> T Cells.

Luff Daisy H DH   Wojdyla Katarzyna K   Oxley David D   Chessa Tamara T   Hudson Kevin K   Hawkins Phillip T PT   Stephens Len R LR   Barry Simon T ST   Okkenhaug Klaus K  

Frontiers in immunology 20210308

Phosphoinositide 3-kinases (PI3Ks) play a central role in adaptive immunity by transducing signals from the T cell antigen receptor (TCR) via production of PIP<sub>3</sub>. PI3Kδ is a heterodimer composed of a p110δ catalytic subunit associated with a p85α or p85β regulatory subunit and is preferentially engaged by the TCR upon T cell activation. The molecular mechanisms leading to PI3Kδ recruitment and activation at the TCR signalosome remain unclear. In this study, we have used quantitative ma  ...[more]

Similar Datasets

Proteomics Time-course phosphoproteome response during adenovirus type 2 infection
2020-07-03 | PXD009562 | Pride
Transcriptomics TCR repertoire profiling reveals HLA-dependent and persistent T-cell expansions in synovial fluid of patients with spondyloarthritis
2023-05-31 | E-MTAB-11498 | biostudies-arrayexpress
Proteomics Mtfp1 ablation enhances mitochondrial respiration and protects against hepatic steatosis- Co-IP
2023-11-27 | PXD046262 | Pride
Transcriptomics Single cell RNA-seq of human CD8+ T cells binding the IAV HLA-A24/PB1 498-505 tetramer
2021-04-08 | E-MTAB-10109 | biostudies-arrayexpress
Transcriptomics Deep sequencing of human naive CD4 T cells identifies miR-34c-5p as a novel regulator of T cell activation and HIV replication
2016-12-21 | E-MTAB-4214 | biostudies-arrayexpress
Genomics Investigating Biofilm Formation Mechanisms in Salmonella on Fresh Produce Using TraDIS-Xpress
2023-11-24 | E-MTAB-13495 | biostudies-arrayexpress
Proteomics Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T cells
2019-10-02 | PXD007988 | Pride
Proteomics importance of MTFP1 partners for mitochondrial function in the heart
2022-10-06 | PXD028529 | Pride
Transcriptomics Phenotypic differences in dopaminergic circuitries of mouse strains are associated with extensive regulatory variation in the midbrain
2019-10-07 | E-MTAB-8333 | biostudies-arrayexpress
Proteomics Comparative Analysis of T Cell Spatial Proteomics and the Influence of HIV Expression
2022-01-16 | PXD029956 | Pride