Proteomics

Dataset Information

0

Adaptive responses of Pseudomonas aeruginosa to treatment with antibiotics and non-steroidal anti-inflammatory drugs

ABSTRACT: Circumventing or overwhelming the bacterial adaptation capabilities is key to combatting multidrug-resistant pathogens like Pseudomonas aeruginosa. We investigated the physiological stress exerted by approved antibiotics (ciprofloxacin, levofloxacin, rifampicin, gentamicin, tobramycin, azithromycin, tigecycline, polymyxin B, colistin, ceftazidime, meropenem, piperacillin/tazobactam), experimental antibiotics (CHIR-090) and NSAIDs (acetylsalicylic acid (aspirin), diclofenac, ibuprofen), and studied the bacterial response on the proteome level. Radioactive pulse-labeling of newly synthesized proteins followed by 2D-PAGE was used to monitor the acute response of P. aeruginosa to antibiotic treatment. Subsequently, marker proteins were excised from non-radioactive gels and identified by mass spectrometry. We generated a reference library of P. aeruginosa proteomic responses and implemented a mathematical comparison of the profiles. Proteomic signatures were derived for clinically relevant target areas.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Pseudomonas Aeruginosa Pao1

SUBMITTER: Dominik Wüllner  

LAB HEAD: Julia Elisabeth Bandow

PROVIDER: PXD022879 | Pride | 2022-08-12

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Adaptive Responses of <i>Pseudomonas aeruginosa</i> to Treatment with Antibiotics.

Wüllner Dominik D   Gesper Maren M   Haupt Annika A   Liang Xiaofei X   Zhou Pei P   Dietze Pascal P   Narberhaus Franz F   Bandow Julia E JE  

Antimicrobial agents and chemotherapy 20211108 1

Pseudomonas aeruginosa is among the highest priority pathogens for drug development because of its resistance to antibiotics, extraordinary adaptability, and persistence. Antipseudomonal research is strongly encouraged to address the acute scarcity of innovative antimicrobial lead structures. In an effort to understand the physiological response of P. aeruginosa to clinically relevant antibiotics, we investigated the proteome after exposure to ciprofloxacin, levofloxacin, rifampicin, gentamicin,  ...[more]

Similar Datasets

Proteomics Adaptive responses of Pseudomonas aeruginosa to treatment with antibiotics
2021-11-29 | PXD029948 | Pride
Transcriptomics Tobramycin Treatment of Planktonic Pseudomonas aeruginosa
2008-06-10 | GSE9991 | GEO
Proteomics Interspecies comparison of the bacterial response to allicin reveals species-specific defense strategies
2021-03-09 | PXD012622 | Pride
Transcriptomics A mouse tumor biofilm model and its use for dissecting in vivo anti-bacterial and anti-biofilm activities of anti-pseudomonas drugs
2019-02-01 | GSE55000 | GEO
Transcriptomics Transcription profiling of planktonic pseudomonas aeruginosa exposed to tobramycin treatment
2008-06-21 | E-GEOD-9991 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Pseudomonas aeruginosa treated with tobramycin
2008-06-18 | E-GEOD-10030 | biostudies-arrayexpress
Genomics Identification of adaptive mechanisms leading to reduced antibiotic susceptibility in bacterial biofilms using experimental evolution. Part 1: Pseudomonas aeruginosa strain AA2 biofilms on beads
2022-08-18 | E-MTAB-12091 | biostudies-arrayexpress
Proteomics Applicability of chromatographic co-elution for antibiotic research
2020-10-01 | PXD017640 | Pride
Transcriptomics RNA sequencing of Pseudomonas aeruginosa phoenix colonies, viable but non-culturable colonies (VBNCs), and control samples
2022-03-27 | GSE199323 | GEO
Genomics Using small RNA-Seq to identify differential small RNA content of bacterial outer membrane vesicles (OMVs) secreted by Pseudomonas aeruginosa in response to tobramycin exposure
2021-09-12 | GSE183895 | GEO