Proteomics

Dataset Information

0

Adaptive responses of Pseudomonas aeruginosa to treatment with antibiotics and non-steroidal anti-inflammatory drugs

ABSTRACT: Circumventing or overwhelming the bacterial adaptation capabilities is key to combatting multidrug-resistant pathogens like Pseudomonas aeruginosa. We investigated the physiological stress exerted by approved antibiotics (ciprofloxacin, levofloxacin, rifampicin, gentamicin, tobramycin, azithromycin, tigecycline, polymyxin B, colistin, ceftazidime, meropenem, piperacillin/tazobactam), experimental antibiotics (CHIR-090) and NSAIDs (acetylsalicylic acid (aspirin), diclofenac, ibuprofen), and studied the bacterial response on the proteome level. Radioactive pulse-labeling of newly synthesized proteins followed by 2D-PAGE was used to monitor the acute response of P. aeruginosa to antibiotic treatment. Subsequently, marker proteins were excised from non-radioactive gels and identified by mass spectrometry. We generated a reference library of P. aeruginosa proteomic responses and implemented a mathematical comparison of the profiles. Proteomic signatures were derived for clinically relevant target areas.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Pseudomonas Aeruginosa Pao1

SUBMITTER: Dominik Wüllner  

LAB HEAD: Julia Elisabeth Bandow

PROVIDER: PXD022879 | Pride | 2022-08-12

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
170315_Cipro1.raw.zip Raw
170315_Cipro10.raw.zip Raw
170315_Cipro11.raw.zip Raw
170315_Cipro12.raw.zip Raw
170315_Cipro13.raw.zip Raw
Items per page:
1 - 5 of 387
altmetric image

Publications

Adaptive Responses of <i>Pseudomonas aeruginosa</i> to Treatment with Antibiotics.

Wüllner Dominik D   Gesper Maren M   Haupt Annika A   Liang Xiaofei X   Zhou Pei P   Dietze Pascal P   Narberhaus Franz F   Bandow Julia E JE  

Antimicrobial agents and chemotherapy 20211108 1

Pseudomonas aeruginosa is among the highest priority pathogens for drug development because of its resistance to antibiotics, extraordinary adaptability, and persistence. Antipseudomonal research is strongly encouraged to address the acute scarcity of innovative antimicrobial lead structures. In an effort to understand the physiological response of P. aeruginosa to clinically relevant antibiotics, we investigated the proteome after exposure to ciprofloxacin, levofloxacin, rifampicin, gentamicin,  ...[more]

Similar Datasets

Proteomics Adaptive responses of Pseudomonas aeruginosa to treatment with antibiotics
2021-11-29 | PXD029948 | Pride
Proteomics Interspecies comparison of the bacterial response to allicin reveals species-specific defense strategies
2021-03-09 | PXD012622 | Pride
Proteomics Applicability of chromatographic co-elution for antibiotic research
2020-10-01 | PXD017640 | Pride
Proteomics Comparison of Proteomic Responses
2020-10-01 | PXD011640 | Pride
Proteomics The natural product elegaphenone potentiates antibiotic effects against Pseudomonas aeruginosa
2021-09-08 | PXD010476 | Pride
Transcriptomics Investigations of the effects of subMIC antibiotics on the P. aeruginosa transcriptome
2008-02-28 | GSE8953 | GEO
Transcriptomics Expression pattern of Pseudomonas aeruginosa biofilm-residing bacteria exposed to gentamicin or surface acoustic waves or gentamicin and surface acoustic waves combined
2011-09-01 | GSE28081 | GEO
Transcriptomics A novel inhibitor of P. aeruginosa folate metabolism exploits metabolic differences for narrow-spectrum antibiotic targeting
2024-02-07 | GSE249862 | GEO
Transcriptomics A mouse tumor biofilm model and its use for dissecting in vivo anti-bacterial and anti-biofilm activities of anti-pseudomonas drugs
2019-02-01 | GSE55000 | GEO
Transcriptomics Epinecidin-1 has immunomodulatory effects, facilitating its therapeutic use in a mouse model of Pseudomonas aeruginosa sepsis
2014-05-27 | GSE57976 | GEO