Proteomics

Dataset Information

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Comparison of Proteomic Responses


ABSTRACT: To facilitate antibiotic research, we have developed a compendium of proteomic responses that allows rapid classification of compounds into those with precedented and unprecedented modes of action. We gathered more than 100 proteomic response profiles of B. subtilis to treatment with antibiotics. To depict acute bacterial responses to sublethal doses of antibiotics, we used pulse-labeling of proteins that are newly synthesized after antibiotic challenge and analyze the proteome by 2D-PAGE. Subsequently, marker proteins were excised from non-radioactive gels and identified by mass spectrometry. Through compilation of response profiles, marker proteins are delineated as signatures, which are specific for inhibition of cellular processes like DNA, RNA, or protein biosynthesis, metabolic processes like fatty acid or folic acid biosynthesis, and attacks on cellular structures like the cytoplasmic membrane or the cell wall.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Sina Schäkermann  

LAB HEAD: Prof. Dr. Julia Bandow

PROVIDER: PXD011640 | Pride | 2020-10-01

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
020217_Ciprofloxacin_1011.raw.rar Raw
020217_Ciprofloxacin_1080.raw.rar Raw
020217_Ciprofloxacin_1092.raw.rar Raw
020217_Ciprofloxacin_1100.raw.rar Raw
020217_Ciprofloxacin_1142.raw.rar Raw
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