Proteomics

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Nucleolin is a cell surface receptor of C1QTNF4

ABSTRACT: C1QTNF4 is a secreted protein that is structurally unique within the C1QTNF family, a family of proteins that have structural homology with both complement C1q and the tumor necrosis factor superfamily. The role of C1QTNF4 in immunity and inflammation remains poorly defined and a cell surface receptor has yet to be identified. Here we performed co-immunoprecipitation with subsequent mass spectrometric analysis in order to identify a cell surface receptor of C1QTNF4. We report identification of nucleolin as a cell surface binding partner of C1QTNF4. Nucleolin may serve as a docking molecule for C1QTNF4 and act in a context-dependent manner through co-receptors. Having identified nucleolin as a cell surface receptor is a further step to understanding C1QTNF4’s function in the healthy immune system.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Susan Vester  

LAB HEAD: Timothy J Vyse

PROVIDER: PXD023088 | Pride | 2021-03-21

REPOSITORIES: Pride

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Action DRS
receptor-coIP_sample1.msf Msf
receptor-coIP_sample1.raw Raw
receptor-coIP_sample2.msf Msf
receptor-coIP_sample2.raw Raw
receptor-coIP_sample3.msf Msf
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Publications

Nucleolin acts as the receptor for C1QTNF4 and supports C1QTNF4-mediated innate immunity modulation.

Vester Susan K SK   Beavil Rebecca L RL   Lynham Steven S   Beavil Andrew J AJ   Cunninghame Graham Deborah S DS   McDonnell James M JM   Vyse Timothy J TJ  

The Journal of biological chemistry 20210101

The C1q and TNF related 4 (C1QTNF4) protein is a structurally unique member of the C1QTNF family, a family of secreted proteins that have structural homology with both complement C1q and the tumor necrosis factor superfamily. C1QTNF4 has been linked to the autoimmune disease systemic lupus erythematosus through genetic studies; however, its role in immunity and inflammation remains poorly defined and a cell surface receptor of C1QTNF4 has yet to be identified. Here we report identification of nu  ...[more]

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