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Secretomic analysis of a novel strain SM1988

ABSTRACT: Strain SM1988T is a Gram-negative, aerobic, oxidase- and catalase-positive, unipolar flagellated, and rod-shaped bacterium capable of hydrolyzing casein, gelatin and collagens. Phylogenetic analysis revealed that strain SM1988T formed a distinct phylogenetic lineage along with known genera within the family Pseudoalteromonadaceae, with 16S rRNA gene sequence similarity being less than 93.3% to all known species in the family. Based on the phylogenetic, genomic, chemotaxonomic and phenotypic data, strain SM1988T was considered to represent a novel species in a novel genus in the family Pseudoalteromonadaceae, for which the name Flocculibacter collagenilyticus gen. nov., sp. nov. is proposed, with the type strain being SM1988T (= MCCC 1K04279T = KCTC 72761T). Strain SM1988T showed a high production (236 U/mL) of extracellular collagenases, which had high activity against both bovine collagen and codfish collagen. Biochemical tests combined with genomic and secretomic analyses indicated that the collagenases secreted by strain SM1988T are serine proteases from the S8 family. These data suggest that strain SM1988T acts as an important player in marine collagen degradation and recycling and may have a promising potential in collagen resource utilization.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Pseudoalteromonas Sp. S-12

TISSUE(S): Cell Culture

SUBMITTER: Jian Li

LAB HEAD: Yuzhong Zhang

PROVIDER: PXD023431 | Pride | 2021-01-14

REPOSITORIES: Pride

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			Action	DRS
	ZYZ_LJ_1988_20200629.raw	Raw
	ZYZ_LJ_1988_20200629_2.txt	Txt

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