Proteomics

Dataset Information

0

Surfaceome and exoproteome analysis of Staphylococcus aureus and Pseudomonas aeruginosa mono- and co-culture biofilms


ABSTRACT: Staphylococcus aureus and Pseudomonas aeruginosa are bacterial pathogens that have been shown to co-exist in biofilms related to numerous infections. Although the interaction between these two species is competitive, both partially benefit from the coexistence. In this study, we exhaustively characterized the interaction between Staphylococcus aureus and Pseudomonas aeruginosa by utilizing a proteomics approach, individually targeting the surface-associated proteins (surfaceome), and proteins secreted or otherwise liberated to the extracellular space (exoproteome). To that end, the conditions to co-culture S. aureus and P. aeruginosa in vitro were optimized and a high-resolution proteomics approach was applied to compare surface-associated and extracellular protein profiles between mono- and co-cultured biofilms.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Tuula Nyman  

LAB HEAD: Tuula Nyman

PROVIDER: PXD023445 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
P14898.raw Raw
P14900.raw Raw
P14902.raw Raw
P14906.raw Raw
P14908.raw Raw
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Publications

Surfaceome and Exoproteome Dynamics in Dual-Species <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> Biofilms.

Reigada Inés I   San-Martin-Galindo Paola P   Gilbert-Girard Shella S   Chiaro Jacopo J   Cerullo Vincenzo V   Savijoki Kirsi K   Nyman Tuula A TA   Fallarero Adyary A   Miettinen Ilkka I  

Frontiers in microbiology 20210625


Bacterial biofilms are an important underlying cause for chronic infections. By switching into the biofilm state, bacteria can evade host defenses and withstand antibiotic chemotherapy. Despite the fact that biofilms at clinical and environmental settings are mostly composed of multiple microbial species, biofilm research has largely been focused on single-species biofilms. In this study, we investigated the interaction between two clinically relevant bacterial pathogens (<i>Staphylococcus aureu  ...[more]

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