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RNAseq of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus USA300 in monocultures and co-cultivated at 37ºC and 39ºC in microaerobiosis

ABSTRACT: Staphylococcus aureus USA300 and Pseudomonas aeruginosa PAO1 were cultured in microaerobiosis (sealed bottles using a 1:4 medium-to-flask volume ratio without agitation) in Triptic Soy Broth (TSB; Oxoid) supplemented with 0.5% KNO3. S. aureus and P. aeruginosa monocultures were inoculated at a DO600 of 0.05 and incubated for 2h at 37°C. After that, cultures were split in two and furtherly incubated for 2h at 37°C (control) or 39°C (heat shock). A similar protocol was employed for co-culture experiments, where S. aureus and P. aeruginosa were co-inoculated each at a DO600 of 0.05 and followed the same protocol.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Staphylococcus aureus subsp. aureus USA300

SUBMITTER: Esmeralda SolarVenero

PROVIDER: E-MTAB-12581 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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