Dataset Information

Specificity of the protase acrosin in zona pellucida proteins

ABSTRACT: This study aims to characterize the sperm protease acrosin.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig) Mus Musculus (mouse)

TISSUE(S): Oocyte

DISEASE(S): Infertility

SUBMITTER: Thomas Michna

LAB HEAD: Stefan Tenzer

PROVIDER: PXD023659 | Pride | 2021-04-09

REPOSITORIES: Pride

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Publications

Limited proteolysis by acrosin affects sperm-binding and mechanical resilience of the mouse zona pellucida.

Kuske Michael M Floehr Julia J Yiallouros Irene I Michna Thomas T Jahnen-Dechent Willi W Tenzer Stefan S Stöcker Walter W Körschgen Hagen H

Molecular human reproduction 20210301 4

The encounter of oocyte and sperm is the key event initiating embryonic development in mammals. Crucial functions of this existential interaction are determined by proteolytic enzymes, such as acrosin, carried in the sperm head acrosome, and ovastacin, stored in the oocyte cortical granules. Ovastacin is released upon fertilisation to cleave the zona pellucida, a glycoprotein matrix surrounding the oocyte. This limited proteolysis hardens the oocyte envelope, and thereby provides a definitive bl ...[more]

PMID: 33779727

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