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Altered glycosylation in the ageing heart

ABSTRACT: Heart failure is one of the leading causes of death in an ageing population. Hallmarks are cardiac hypertrophy, fibrosis and inflammation. The molecular mechanisms, however, are poorly understood. Glycosylation is one of the most common posttranslational modifications of proteins, which can have important consequences for protein folding, function and turnover. We hypothesized that changes in glycoprotein abundance and glycosylation patterns may contribute to cardiac aging. Western Blot analysis suggests increased protein mannosylation in the aging heart. Glycoprotein pull-downs from heart lysates of young (3 months) and old (2 years) mice in combination with quantitative mass spectrometry support widespread alterations of the glycoproteome in aged hearts.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Emilio Cirri

LAB HEAD: Christian Huebner

PROVIDER: PXD023740 | Pride | 2021-05-12

REPOSITORIES: Pride

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			Action	DRS
	201020_EC_NR_A42_WT1_12_M_C_19.raw	Raw
	201020_EC_NR_A42_WT1_12_M_P_7.raw	Raw
	201020_EC_NR_A42_WT1_12_Man_C_22.raw	Raw
	201020_EC_NR_A42_WT1_12_Man_P_10.raw	Raw
	201020_EC_NR_A42_WT1_13_w_C_13.raw	Raw

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