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SNAPIN regulates β cell cycle progression to promote pancreatic β cell growth

ABSTRACT: SNAPIN for β cell growth is poorly understood, and our findings reveal that SNAPIN overexpression in Min6 cells can promote cell proliferation and are promising in achieving the goals of regenerative medicine for diabetes treatment.we want to find snapin interact proteins to illustrate snapin function.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Jiang mengxue

LAB HEAD: Mengxue Jiang

PROVIDER: PXD023786 | Pride | 2021-06-03

REPOSITORIES: Pride

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			Action	DRS
	SNAPIN.msf	Msf
	SNAPIN.raw	Raw
	checksum.txt	Txt

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Project description:In this study, we investigated the molecular and cellular mechanisms of verapamil treatment on β-cell function and survival using MIN6 cells. Verapamil's molecular processes were studied using transcriptomic data. MTT, cell count, and flow cytometry assessed cell proliferation, growth, and cycle. MIN6 cell function was assessed by glucose-stimulated insulin release and total insulin content. Seahorse and metabolic stress kits examined metabolic activity and oxygen consumption rate. The protective impact of verapamil on MIN6 cells was evaluated by challenging the verapamil treated cell with streptozotocin, T1D-cytomix, or T2D-cytomix cocktail. Our results demonstrate that verapamil treatment induced higher proliferation of MIN6 cells, altered expression of various proteins and genes, improved insulin secretion in hyperglycemic conditions, increased basal and maximal respiration levels, along with β-cell survival against streptozotocin, T1D-, or T2D-cytomix-induced toxicity, and rendered a protective effect.

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