Proteomics

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Identification of RON13 binding with with RON4 substrate

ABSTRACT: Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction (MJ), which joins parasite and host cell plasma membranes during entry. Invading Toxoplasma gondii tachyzoites secrete a complex of RON proteins from the rhoptry organelles that provide the components of the MJ on the cytoplasmic side of the host cell membrane. This study highlights the role of a rhoptry resident kinase, RON13, that plays a crucial role during host cell entry and is a virulence factor in the mouse model of infection. Comparative phosphoproteomics demonstrated RON13-dependent phosphorylation of secreted rhoptry proteins and primarily the RONs complex. Structural and biochemical analysis of RON13 revealed the atypical nature of this active secretory pathway kinase, unique to the Apicomplexa.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Oscar Vadas  

LAB HEAD: Dominique Soldati-Favre

PROVIDER: PXD023791 | Pride | 2021-05-10

REPOSITORIES: Pride

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Action DRS
20190820_OT_HDXMS84.mgf Mgf
20190820_OT_HDXMS84.raw Raw
20200129_OT_HDX_050.raw Raw
20200129_OT_HDX_052.raw Raw
20200129_OT_HDX_054.raw Raw
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Publications

Active host cell invasion by the obligate intracellular apicomplexan parasites relies on the formation of a moving junction, which connects parasite and host cell plasma membranes during entry. Invading Toxoplasma gondii tachyzoites secrete their rhoptry content and insert a complex of RON proteins on the cytoplasmic side of the host cell membrane providing an anchor to which the parasite tethers. Here we show that a rhoptry-resident kinase RON13 is a key virulence factor that plays a crucial ro  ...[more]

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