Dataset Information

Fractionation of Naja ashei venom with Size-exclusion chromatography and identification of proteins in obtained fractions

ABSTRACT: The study aimed to fractionate Naja ashei venom with Size-exclusion chromatography to obtain fractions with significantly lower complexity than crude venom. MS analysis allowed to estimate the percentage of different protein groups in each fraction, thus providing information about the extent of homogeneity in each collected sample. High purity fractions can be used to study the effects of particular proteins on different biological targets. Samples with greater complexity can be further purified using different analytical methods.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Naja Ashei

TISSUE(S): Venom

SUBMITTER: Konrad Hus

LAB HEAD: Aleksandra Bocian

PROVIDER: PXD023801 | Pride | 2021-09-10

REPOSITORIES: Pride

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Publications

Effects of 3FTx Protein Fraction from <i>Naja ashei</i> Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity.

Dyba Barbara B Rudolphi-Szydło Elżbieta E Barbasz Anna A Czyżowska Agnieszka A Hus Konrad Kamil KK Petrilla Vladimír V Petrillová Monika M Legáth Jaroslav J Bocian Aleksandra A

Molecules (Basel, Switzerland) 20210409 8

Three-finger toxins are naturally occurring proteins in Elapidae snake venoms. Nowadays, they are gaining popularity because of their therapeutic potential. On the other hand, these proteins may cause undesirable reactions inside the body's cells. A full assessment of the safety of <i>Naja ashei</i> venom components for human cell application is still unknown. The aim of the study was to determine the effect of the exogenous application of three-finger toxins on the cells of monocytes (U-937) an ...[more]

PMID: 33918763

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Project description:The venom apparatus is a conserved organ in parasitoid wasps that shows adaptations correlated with life-style diversification. Recent venom analyses from selected species reveal considerable complexity and high diversity in venom composition existing not only between closely related species but even between strains and individuals, which may partly determine the potential for parasitoid adaptation. However, the investigations have paid little attention to secondary venom components that also have significant functions in parasitism, and the data in regard to full and accurate quantity of venom compositions at the protein level is not available. Using a combination of transcriptomic and label-free quantitative proteomic, we here explored the venom components of the endoparasitoid Tetrastichus brontispae (Eulophidae), a species devoid of polydnavirus, and provided an in-depth comparison of the venom proteomes between its two closely related strains, Tb-On and Tb-Bl. Results showed that approximately 1505 venom proteins were identified in the venom apparatus of T. brontispae, consistent with the classical venom protein characteristics, including enzymes, protease inhibitors, binding proteins and some immune related proteins. The venom extracts also contained novel venom proteins, such as kynurenine-oxoglutarate transaminase, 4-coumarate CoA ligase and venom protein r-like protein. Comparative venom proteomes revealed that significant quantitative and qualitative changes in venom composition occurred when Tb-Bl strain, with an invasive beetle Brontispae longissima pupa as its habitual host, was exposed to another invasive beetle Octodonta nipae pupa as host consecutively for two years; although the most abundant venom proteins were shared between them. These significantly differentially expressed proteins were mainly enriched in fatty acid biosynthesis and melanotic encapsulation response by enrichment analyses. Furthermore, most of the significantly enriched proteins presented strikingly increased levels or were exclusively identified in the Tb-On strain. These combined results indicated that virulence factors in Tb-On strain might be linked to lipid metabolism or more venom is required to inhibit O. nipae pupa’s melanotic encapsulation upon its parasitism. Altogether, our data reveal that venom composition can quickly evolve and respond to host selection, mainly through rapid changes in regulation of protein abundance and/or the emergence of multigenic families by gene duplication. Our data additionally provide invaluable data for further functional analysis of parasitoid venoms.

			Action	DRS
	FH_SEC_KH_PR2.raw	Raw
	Naja_ashei_SEC_Fraction_Ident.xlsx	Xlsx
	SEC_FrakcjaA.raw	Raw
	SEC_FrakcjaB.raw	Raw
	SEC_FrakcjaC.raw	Raw