Dataset Information

Ganaspis hookeri (strain GhFl, formerly 'G1') venom gland proteome

ABSTRACT: The purpose of this project was to identify venom proteins from the venom gland of Ganaspis hookeri (strain GhFl, formerly 'G1'), a parasitoid wasp species that infects fruit flies in the genus Drosophila.

OTHER RELATED OMICS DATASETS IN: PRJNA189658

INSTRUMENT(S): LTQ XL

ORGANISM(S): Ganaspis

TISSUE(S): Venom

SUBMITTER: Todd Schlenke

LAB HEAD: Todd A Schlenke

PROVIDER: PXD023825 | Pride | 2021-01-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	checksum.txt	Txt
	jm_20110602_LTQ_Schlenke_G1_1.RAW	Raw
	jm_20110602_LTQ_Schlenke_G1_2.RAW	Raw
	jm_20110602_LTQ_Schlenke_G1_3.RAW	Raw
	jm_20110602_LTQ_Schlenke_G1_4.RAW	Raw

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Publications

Parasitoid wasp venom SERCA regulates Drosophila calcium levels and inhibits cellular immunity.

Mortimer Nathan T NT Goecks Jeremy J Kacsoh Balint Z BZ Mobley James A JA Bowersock Gregory J GJ Taylor James J Schlenke Todd A TA

Proceedings of the National Academy of Sciences of the United States of America 20130520 23

Because parasite virulence factors target host immune responses, identification and functional characterization of these factors can provide insight into poorly understood host immune mechanisms. The fruit fly Drosophila melanogaster is a model system for understanding humoral innate immunity, but Drosophila cellular innate immune responses remain incompletely characterized. Fruit flies are regularly infected by parasitoid wasps in nature and, following infection, flies mount a cellular immune r ...[more]

PMID: 23690612

Similar Datasets

Project description:The venom apparatus is a conserved organ in parasitoid wasps that shows adaptations correlated with life-style diversification. Recent venom analyses from selected species reveal considerable complexity and high diversity in venom composition existing not only between closely related species but even between strains and individuals, which may partly determine the potential for parasitoid adaptation. However, the investigations have paid little attention to secondary venom components that also have significant functions in parasitism, and the data in regard to full and accurate quantity of venom compositions at the protein level is not available. Using a combination of transcriptomic and label-free quantitative proteomic, we here explored the venom components of the endoparasitoid Tetrastichus brontispae (Eulophidae), a species devoid of polydnavirus, and provided an in-depth comparison of the venom proteomes between its two closely related strains, Tb-On and Tb-Bl. Results showed that approximately 1505 venom proteins were identified in the venom apparatus of T. brontispae, consistent with the classical venom protein characteristics, including enzymes, protease inhibitors, binding proteins and some immune related proteins. The venom extracts also contained novel venom proteins, such as kynurenine-oxoglutarate transaminase, 4-coumarate CoA ligase and venom protein r-like protein. Comparative venom proteomes revealed that significant quantitative and qualitative changes in venom composition occurred when Tb-Bl strain, with an invasive beetle Brontispae longissima pupa as its habitual host, was exposed to another invasive beetle Octodonta nipae pupa as host consecutively for two years; although the most abundant venom proteins were shared between them. These significantly differentially expressed proteins were mainly enriched in fatty acid biosynthesis and melanotic encapsulation response by enrichment analyses. Furthermore, most of the significantly enriched proteins presented strikingly increased levels or were exclusively identified in the Tb-On strain. These combined results indicated that virulence factors in Tb-On strain might be linked to lipid metabolism or more venom is required to inhibit O. nipae pupa’s melanotic encapsulation upon its parasitism. Altogether, our data reveal that venom composition can quickly evolve and respond to host selection, mainly through rapid changes in regulation of protein abundance and/or the emergence of multigenic families by gene duplication. Our data additionally provide invaluable data for further functional analysis of parasitoid venoms.