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Spruce dehydrins analysis by LC-MS/MS

ABSTRACT: Dehydrins (DHNs) are plant-specific proteins that accumulate during the abiotic stress that cause cellular dehydration, such as drought, salinity and freezing. How the dehydrin system in conifers reacts to more short-term drought stress is much less studied. Therefore, we studied the dynamics of dehydrin system in seedlings of Scots pine and Norway spruce both on mRNA and on protein levels under conditions of polyethylene glycole 6000 (PEG)-induced water deficit of different intensities.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Picea Abies

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Igor Fesenko

LAB HEAD: Igor Fesenko

PROVIDER: PXD023962 | Pride | 2021-03-03

REPOSITORIES: pride

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Project description:The purpose of this experiment was to do investigatge the transcriptional effect of anti-TNF treatment in patients suffering from rheumatoid arthritis. Two series of hybridizations were performed. Each hybridization was done with a technical replicate (the same amount of RNA taken from the same amplified RNA aliquot labeled in two separate reactions and hybridized onto two separate arrays). In the first series of hybridizations RNA exptracted from biopsies taken before treatment was hybridized in a common reference design to enable comparisons between patients. In the second series RNA from biopsies taken after and before treatment were hybridized on the same chip in a direct design to enable investigation of the effect of treatment. 10 patients (8 females and 2 males, median age 54, range 25-69) meeting the American College of Rheumatology (ACR) criteria for RA were recruited for this study. All patients received infliximab (an antibody directed against TNF-alpha) infusions 3mg/kg at week 0 and than after 2 weeks and 6 weeks. Five of these patients were receiving prednisolon and all ten patients were treated with methotrexate to a maximum of 20 mg per week. Synovial biopsies were obtained during arthroscopy from all patients before and after a median of 9 weeks of treatment (with one exception, patient 7 where the second biopsy was obtained during open surgery). Biopsies were taken at the site of inflammation close to cartilage (cc) or not close to cartilage (ncc), defined as less or more than 1.5 cm away from cartilage, respectively. One biopsy was taken before and after treatment from patient 5, 7, 9 and 10. Multiple biopsies were retrieved before and/or after treatment from patient 1, 2, 3, 4, 6 and 7. Due to small amounts of RNA available for patient 9 it was only used in the second series of hybridizations. The average biopsy weight was 22 mg. The ethical committee at the Karolinska Hospital approved all experiments on human cells and tissues. Informed consent was obtained from all study subjects.

			Action	DRS
	20200513_002_ZR_AK-1.raw	Raw
	20200513_004_ZR_AK-2.raw	Raw
	checksum.txt	Txt
	results.zip	Other

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Spruce dehydrins analysis by LC-MS/MS

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