Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: Süleyman Bozkurt
LAB HEAD: Christian Münch
PROVIDER: PXD024054 | Pride | 2021-12-18
REPOSITORIES: Pride
Action | DRS | |||
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20210121_MAA_SB_SB57_MitoPrep.raw | Raw | |||
20210121_MAA_SB_SB57_WholeCellProteom.raw | Raw | |||
SEARCH.zip | Other | |||
allSpectra.HCD.FTMS.iso_0.apl | Other | |||
allSpectra.HCD.FTMS.iso_1.apl | Other |
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Molecular cell 20211129 2
Most mitochondrial proteins are translated in the cytosol and imported into mitochondria. Mutations in the mitochondrial protein import machinery cause human pathologies. However, a lack of suitable tools to measure protein uptake across the mitochondrial proteome has prevented the identification of specific proteins affected by import perturbation. Here, we introduce mePROD<sup>mt</sup>, a pulsed-SILAC based proteomics approach that includes a booster signal to increase the sensitivity for mito ...[more]