Dataset Information

Long non-coding RNA Neat1 is a key of translational regulator in hypoxia

ABSTRACT: Internal ribosome entry sites (IRESs) drive translation initiation during stress. In response to hypoxia, (lymph)angiogenic factors responsible for tissue revascularization in ischemic diseases are induced by the IRES-dependent mechanism. Here we searched for IRES trans-acting factors (ITAFs) active in early hypoxia in mouse cardiomyocytes. Using knock-down and proteomics approaches, we show a link between a stressed-induced nuclear body, the paraspeckle, and IRES-dependent translation. Our data reveal that the long non-coding RNA Neat1, an essential paraspeckle component, is a key translational regulator, active on IRESs of (lymph)angiogenic and cardioprotective factor mRNAs. In addition, paraspeckle proteins p54nrb and PSPC1 as well as nucleolin and Rps2, two p54nrb-interacting proteins identified by mass spectrometry, are ITAFs for IRES subgroups. Paraspeckle thus appears as the site of IRESome assembly in the nucleus. Polysome PCR array showed that the Neat1_2 isoform widely affects translation of mRNAs containing IRESs, involved in stress response, angiogenesis or cardioprotection.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Cardiac Muscle Cell

DISEASE(S): Myocardial Ischemia

SUBMITTER: Carine Froment

LAB HEAD: Anne-Catherine Prats

PROVIDER: PXD024067 | Pride | 2023-01-03

REPOSITORIES: Pride

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Long non-coding RNA <i>Neat1</i> and paraspeckle components are translational regulators in hypoxia.

Godet Anne-Claire AC Roussel Emilie E David Florian F Hantelys Fransky F Morfoisse Florent F Alves Joffrey J Pujol Françoise F Ader Isabelle I Bertrand Edouard E Burlet-Schiltz Odile O Froment Carine C Henras Anthony K AK Vitali Patrice P Lacazette Eric E Tatin Florence F Garmy-Susini Barbara B Prats Anne-Catherine AC

eLife 20221222

Internal ribosome entry sites (IRESs) drive translation initiation during stress. In response to hypoxia, (lymph)angiogenic factors responsible for tissue revascularization in ischemic diseases are induced by the IRES-dependent mechanism. Here, we searched for IRES <i>trans</i>-acting factors (ITAFs) active in early hypoxia in mouse cardiomyocytes. Using knock-down and proteomics approaches, we show a link between a stressed-induced nuclear body, the paraspeckle, and IRES-dependent translation. ...[more]

PMID: 36546462

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