Dataset Information

Proteomics on sera of mice following local high-dose heart irradiation

ABSTRACT: Background and Purpose: Cardiotoxicity is a well-known adverse effect of radiation therapy. Measurable abnormalities in the heart function indicate advanced and often irreversible heart damage. Therefore, early detection of cardiac toxicity is necessary to delay and alleviate the development of the disease. The present study investigated long-term serum proteome alterations following local heart irradiation using a mouse model with the aim to detect biomarkers of radiation-induced cardiac toxicity. Materials and Methods: Serum samples from C57BL/6J mice were collected 20 weeks after local heart irradiation with 8 Gy or 16 Gy X-ray; the controls were sham-irradiated. The samples were analyzed by quantitative proteomics based on data-independent acquisition mass spectrometry. The proteomics data were further investigated using bioinformatics and ELISA. Results: The analysis showed radiation-induced changes in the level of several serum proteins involved in the acute phase response, inflammation and cholesterol metabolism. We found significantly enhanced expression of pro-inflammatory cytokines (TNF-, TGF-, IL-1 and IL-6) in the serum of the irradiated mice. The level of free fatty acids, total cholesterol, low density lipoprotein (LDL) and oxidized LDL was increased whereas that of high density lipoprotein was decreased by irradiation. Conclusions: This study provides information on systemic effects of heart irradiation. It elucidates a radiation fingerprint in the serum that may be used to elucidate adverse cardiac effects after radiation therapy.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Serum

SUBMITTER: Christine von Toerne

LAB HEAD: Soile Tapio

PROVIDER: PXD024446 | Pride | 2021-09-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	039_mouse_meta_speclib_21childsteps_April2017.kit	Other
	210215_CVT10026_Serum_0Gy_8Gy_16Gy_DIA_AnalysisProteinReport.xlsx	Xlsx
	CVT10026A10MA2__010-110_Wild_8_Gy_CVT10026A10MA2-40-356928.raw	Raw
	CVT10026A11MA2__011-68_Wild_8_Gy_CVT10026A11MA2-40-356929.raw	Raw
	CVT10026A12MA2__012-91_Wild_16_Gy_CVT10026A12MA2-40-356930.raw	Raw

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Publications

Data-Independent Acquisition Proteomics Reveals Long-Term Biomarkers in the Serum of C57BL/6J Mice Following Local High-Dose Heart Irradiation.

Azimzadeh Omid O von Toerne Christine C Subramanian Vikram V Sievert Wolfgang W Multhoff Gabriele G Atkinson Michael J MJ Tapio Soile S

Frontiers in public health 20210702

<b>Background and Purpose:</b> Cardiotoxicity is a well-known adverse effect of radiation therapy. Measurable abnormalities in the heart function indicate advanced and often irreversible heart damage. Therefore, early detection of cardiac toxicity is necessary to delay and alleviate the development of the disease. The present study investigated long-term serum proteome alterations following local heart irradiation using a mouse model with the aim to detect biomarkers of radiation-induced cardiac ...[more]

PMID: 34277544

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