Proteomics

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LC-MS/MS of the reaction center-light harvesting 1 complex from Gemmatimonas phototrophica

ABSTRACT: Gemmatimonas phototrophica is the only phototrophic member of the recently discovered bacterial phylum Gemmatimonadetes. It was isolated from a freshwater lake in the Gobi desert and first described in 2014. So far, Gemmatimonas phototrophica is the only bacterium to have received a complete set of photosynthesis-related genes by horizontal gene transfer from an ancient phototrophic species from the phylum Proteobacteria. This organism illustrates the possibility for engineering phototrophic capability in a non-phototrophic organism and is therefore of great interest to the field of synthetic biology. The structure of the photosynthetic reaction center-light harvesting 1 complex is under investigation by cryo-EM. Proteomic analysis verified the identities of the expected protein components of this complex and, additionally revealed polypeptides that were previously undiscovered and could be mapped to the cryo-EM images.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Gemmatimonas Phototrophica

TISSUE(S): Photosynthetic Cell

SUBMITTER: Philip Jackson  

LAB HEAD: Pu Qian

PROVIDER: PXD024609 | Pride | 2022-04-04

REPOSITORIES: Pride

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20200703_Gemm-phot_PS_pepsin_500ng_1.raw Raw
20200703_Gemm-phot_PS_pepsin_500ng_1.raw_20210308_Byonic.xlsx Xlsx
20200703_Gemm-phot_PS_pepsin_500ng_2.raw Raw
20200703_Gemm-phot_PS_pepsin_500ng_2.raw_20210308_Byonic.xlsx Xlsx
20200703_Gemm-phot_PS_pepsin_500ng_3.raw Raw
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Publications

Phototrophic Gemmatimonadetes evolved the ability to use solar energy following horizontal transfer of photosynthesis-related genes from an ancient phototrophic proteobacterium. The electron cryo-microscopy structure of the <i>Gemmatimonas phototrophica</i> photosystem at 2.4 Å reveals a unique, double-ring complex. Two unique membrane-extrinsic polypeptides, RC-S and RC-U, hold the central type 2 reaction center (RC) within an inner 16-subunit light-harvesting 1 (LH1) ring, which is encircled b  ...[more]

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