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1,2-Propanediol utilization mediated by bacterial microcompartments in Propionibacterium freudenreichii DSM 20271

ABSTRACT: This study was conducted to investigate whether 1,2-propanediol could be metabolized by Propionibacterium freudenreichii DSM 20271 through bacterial microcompartment mediated metabolism.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Propionibacterium Freudenreichii

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD024700 | Pride | 2021-03-18

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	MQresult_AlexTA185-190.zip	Other
	TA185_Lac_RefA.raw	Raw
	TA186_Lac_RefB.raw	Raw
	TA187_Lac_RefC.raw	Raw
	TA188_PD_A.raw	Raw

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Publications

Bacterial Microcompartment-Dependent 1,2-Propanediol Utilization of <i>Propionibacterium freudenreichii</i>.

Dank Alexander A Zeng Zhe Z Boeren Sjef S Notebaart Richard A RA Smid Eddy J EJ Abee Tjakko T

Frontiers in microbiology 20210512

Bacterial microcompartments (BMCs) are proteinaceous prokaryotic organelles that enable the utilization of substrates such as 1,2-propanediol and ethanolamine. BMCs are mostly linked to the survival of particular pathogenic bacteria by providing a growth advantage through utilization of 1,2-propanediol and ethanolamine which are abundantly present in the human gut. Although a 1,2-propanediol utilization cluster was found in the probiotic bacterium <i>Propionibacterium freudenreichii</i>, BMC-med ...[more]

PMID: 34054787

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Project description:This study was conducted in order to monitor whether or not Listeria monocytogenes is able to form bacterial microcompartment for rhamnose metabolism by deploying 1,2-propanediol bacterial microcompartment shell proteins and encapsulated enzymes.

2021-06-21 | PXD025734 | Pride

Expression analysis of strains Lactococcus lactis KCTC 3769T, L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T

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Gene expression comparison of L. reuteri DSM 17938 and pocR mutant

Project description:Comparison of gene expression between L. reuteri DSM 17938 and L. reuteri DSM 17938::pocR mutant grown in semi-defined medium after 24h of growth at 37C in anaerobic condition. PocR is an AraC-like transcriptional regulator, and changes in gene expression between mutant and wild-type strains would indicate genes involved in the PocR regulon. Includes 3 biological replicates and dye-swaps for DSM 17938 versus pocR mutant. One sample includes total RNA isolated from wildtype DSM 17938 labeled with either cy3 or cy5, and total RNA isolated from the pocR mutant labeled with the opposite dye. Samples 1, 2, and 3 represent biological replicates. Samples 4, 5, and 6 represent dye-swaps of the same biological replicates.

2014-01-24 | E-GEOD-54324 | biostudies-arrayexpress

Project description:Investigation of whole genome gene expression level changes in Lactococcus lactis KCTC 3769T,L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T . This proves that transcriptional profiling can facilitate in elucidating the genetic distance between closely related strains. A one chip study using total RNA recovered from of L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T . For the the transcriptome of of L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T was analyzed using the Lactococcus lactis KCTC 3769T microarray platform

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Aerobic Adaptation and Metabolic Dynamics of Propionibacterium freudenreichii DSM 20271: Insights from Comparative Transcriptomics and Surfaceome Analysis

Project description:Propionibacterium freudenreichii (PFR) DSM 20271 is a bacterium known for its ability to thrive in diverse environments and produce vitamin B12. Despite its anaerobic preference, recent studies have elucidated its ability to prosper in the presence of oxygen, prompting a deeper exploration of its physiology under aerobic conditions. Here, we investigated the response of DSM 20271 to aerobic growth by employing comparative transcriptomic and surfaceome analyses alongside metabolite profiling. Cultivation under controlled partial pressure of oxygen (pO2) conditions revealed significant increases in biomass formation and altered metabolite production, notably B12 vitamin, pseudovitamin-B12, propionate and acetate, under aerobic conditions. Transcriptomic analysis identified differential expression of genes involved in lactate metabolism, TCA cycle, and electron transport chain, suggesting metabolic adjustments to aerobic environments. Moreover, surfaceome analysis unveiled growth environment-dependent changes in surface protein abundance, with implications for sensing and adaptation to atmospheric conditions. Supplementation experiments with key compounds highlighted the potential for enhancing aerobic growth, emphasizing the importance of iron and α-ketoglutarate availability. Furthermore, in liquid culture, FeSO4 supplementation led to increased heme production and reduced vitamin B12 production, highlighting the impact of oxygen and iron availability on the metabolic pathways. These findings deepen our understanding of PFR's physiological responses to oxygen availability and offer insights for optimizing its growth in industrial applications.

2024-09-09 | PXD051703 | Pride

Gene expression comparison of L. reuteri DSM 17938 and pocR mutant

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