Dataset Information

Ethylene glycol metabolism in Propionibacterium freudenreichii

ABSTRACT: This study was performed to monitor ethylene glycol metabolism by Propionibacterium freudenreichii and to elucidate the metabolic pathway involved.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Propionibacterium Freudenreichii

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD037369 | Pride | 2024-08-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MQresult_TA397-402.zip	Other
	TA397_Lactate_grown_1.raw	Raw
	TA398_Lactate_grown_2.raw	Raw
	TA399_Lactate_grown_3.raw	Raw
	TA400_Ethylene-glycol_gr_1.raw	Raw

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Publications

Ethylene glycol is metabolized to ethanol and acetate and induces expression of bacterial microcompartments in <i>Propionibacterium freudenreichii</i>.

Dank Alexander A Liu Yue Y Wen Xin X Lin Fan F Wiersma Anne A Boeren Sjef S Smid Eddy J EJ Notebaart Richard A RA Abee Tjakko T

Heliyon 20240622 13

Ethylene glycol (EG, 1,2-ethanediol) is a two-carbon dihydroxy alcohol that can be derived from fermentation of plant-derived xylose and arabinose and which can be formed during food fermentations. Here we show that <i>Propionibacterium freudenreichii</i> DSM 20271 is able to convert EG in anaerobic conditions to ethanol and acetate in almost equimolar amounts. The metabolism of EG led to a moderate increase of biomass, indicating its metabolism is energetically favourable. A proteomic analysis ...[more]

PMID: 39027605

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Project description:Propionibacterium freudenreichii is used as a ripening culture in Swiss cheese manufacture. It produces flavor compounds over the whole ripening period. During cheese ripening, P. freudenreichii is exposed to a temperature downshift, especially when cheeses are transferred from warm temperature (about 24°C) to cold temperature (about 4°C). The aim of this study was to investigate the adaptation of P. freudenreichii at cold temperature by means of the first global gene expression profile for this species. The temporal transcriptomic response of P. freudenreichii was analyzed at five times of growth, during growth at 30°C then for 9 days at 4°C, in the constant presence of lactate as the main carbon source. P. freudenreichii response was also investigated by RT-qPCR for 30 genes, by proteomics and metabolomics (main metabolites quantified in culture supernatant). Microarray analysis revealed that 565 genes (25% of the protein-coding sequences of P. freudenreichii genome) were differentially expressed during transition from warm to cold temperature (P < 0.05 and |fold change| > 1). Most of the down-expressed genes were involved in cell machinery (cell division, protein turnover, translation, transcription and DNA replication). During incubation at cold temperature, P. freudenreichii accumulated carbon supplies by up-regulating genes involved in lactate, alanine and serine conversion to pyruvate, in gluconeogenesis and in glycogen synthesis. Interestingly, some genes involved in the formation of important flavor compounds of cheese, coding for an extracellular lipolytic esterases and enzymes of the pathways of formation of branched-chain compounds, were not significantly affected by cold. In conclusion, P. freudenreichii is metabolically active at cold temperature and induces pathways to maintain its long-term viability, which could explain its contribution to cheese ripening even at low temperature. Gene expression was measured in the middle of exponential growth phase at 30°C (20 h, OD650 ≈ 0.5), at the end of exponential growth phase (40 h, OD650 ≈ 2), and after 3, 6 and 9 days of incubation at 4°C. Three independent biological experiments were performed at each time (20h, 40h, 3, 6 and 9 days) and labelled A, B, C. One technical repetition was performed using the RNA of the 20HA sample ; This technical repetition was labelled 20HAbis.