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Ethylene glycol metabolism in Propionibacterium freudenreichii

ABSTRACT: This study was performed to monitor ethylene glycol metabolism by Propionibacterium freudenreichii and to elucidate the metabolic pathway involved.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Propionibacterium Freudenreichii

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD037369 | Pride | 2024-08-09

REPOSITORIES: Pride

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			Action	DRS
	MQresult_TA397-402.zip	Other
	TA397_Lactate_grown_1.raw	Raw
	TA398_Lactate_grown_2.raw	Raw
	TA399_Lactate_grown_3.raw	Raw
	TA400_Ethylene-glycol_gr_1.raw	Raw

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Publications

Ethylene glycol is metabolized to ethanol and acetate and induces expression of bacterial microcompartments in <i>Propionibacterium freudenreichii</i>.

Dank Alexander A Liu Yue Y Wen Xin X Lin Fan F Wiersma Anne A Boeren Sjef S Smid Eddy J EJ Notebaart Richard A RA Abee Tjakko T

Heliyon 20240622 13

Ethylene glycol (EG, 1,2-ethanediol) is a two-carbon dihydroxy alcohol that can be derived from fermentation of plant-derived xylose and arabinose and which can be formed during food fermentations. Here we show that <i>Propionibacterium freudenreichii</i> DSM 20271 is able to convert EG in anaerobic conditions to ethanol and acetate in almost equimolar amounts. The metabolism of EG led to a moderate increase of biomass, indicating its metabolism is energetically favourable. A proteomic analysis ...[more]

PMID: 39027605

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Insights on cryoprotectant toxicity from gene expression profiling of endothelial cells exposed to ethylene glycol

Project description:Cryopreservation consists of preserving living cells or tissues at <-100˚C and has many applications in, for instance, stem cell and organ banking. Cryoprotectant agents, like ethylene glycol, are required for successful cryopreservation but have toxic side effects due to largely unknown mechanisms. In this work, we studied the toxicity of ethylene glycol in Human Umbilical Vein Endothelial Cells (HUVECs). Exposing cells to 60% ethylene glycol for two hours at 4˚C resulted in a slight decrease in cell growth, suggesting a modest toxicity of ethylene glycol and that HUVECs do not exhibit particular sensitivity to it. Gene expression analysis with whole genome micro-arrays revealed signatures indicative of a generalized stress response at 24 hours after stress and recovery at 72 hours, involving signaling pathways, glycoproteins, and genes involved in extracellular and transmembrane functions. These results reveal a new paradigm and signatures for future experiments in elucidating the toxicity effects of ethylene glycol in vascular endothelial cells.

2015-11-30 | GSE67511 | GEO

Ethylene glycol improves electrospray ionization

Project description:We evaluated a number of compounds as LC additives to modify the electrospray response and found that ethylene glycol was capable of enhancing nESI response by a factor of twofold on average. This result was comparable to DMSO.

2017-07-14 | PXD004919 | Pride

Ethylene treated Arabidopsis roots

Project description:Flooded plants experience impaired gas diffusion underwater, leading to oxygen deprivation (hypoxia) stress. The volatile plant hormone ethylene is rapidly trapped in submerged plant cells and is instrumental for enhanced metabolic hypoxia acclimation. However, the precise mechanisms underpinning ethylene-enhanced hypoxia survival remain unclear. We studied the effect of ethylene pre-treatment on hypoxia survival of primary Arabidopsis thaliana root tips.

2022-12-19 | PXD031138 | Pride

Histidine-Kinase Activity of the Ethylene Receptor ETR1 Facilitates the Ethylene Response in Arabidopsis

Project description:Ethylene response in Arabidopsis with wild-type or kinase-inactive ETR1

2012-04-01 | E-MEXP-3574 | biostudies-arrayexpress

Identification of rice ethylene-response mutants and characterization of MHZ7 in distinct ethylene response and yield trait regulation

Project description:Ethylene plays major roles in adaptive growth of rice plants in water-saturated soil; however, ethylene signaling in rice is largely unclear. Here, we report identification and characterization of ethylene-response mutants based on distinct ethylene-response phenotypes of dark-grown rice seedlings. The seeds were soaked in tap-water and germinated at 37M-BM-0C in the dark for two days (change the water daily). For ethylene treatment, we reduced the amount of water to 1.5-1.7cm below the seeds.Three-day old seedlings were treated with ethylene (10 ppm) for 8 hours at 28M-BM-0C in the dark. Seedling grown in the air was used as control. We isolated RNA from roots and shoots of wild-type and mutant.

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Identification of rice ethylene-response mutants and characterization of mhz2 and mhz8 in distinct ethylene response and yield trait regulation

Project description:Ethylene plays major roles in adaptive growth of rice plants in water-saturated soil; however, ethylene signaling in rice is largely unclear. Here, we report identification and characterization of ethylene-response mutants based on distinct ethylene-response phenotypes of dark-grown rice seedlings. The seeds were soaked in tap-water and germinated at 37Â°C in the dark for two days (change the water daily). For ethylene treatment, we reduced the amount of water to 1.5-1.7cm below the seeds.Three-day old seedlings were treated with ethylene (10 ppm) for 8 hours at 28Â°C in the dark. Seedling grown in the air was used as control. We isolated RNA from roots and shoots of wild-type and mutant.

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Identification of rice ethylene-response mutants and characterization of mhz1, mhz4 and mhz5 in distinct ethylene response and yield trait regulation

Project description:Ethylene plays major roles in adaptive growth of rice plants in water-saturated soil; however, ethylene signaling in rice is largely unclear. Here, we report identification and characterization of ethylene-response mutants based on distinct ethylene-response phenotypes of dark-grown rice seedlings. The seeds were soaked in tap-water and germinated at 37Â°C in the dark for two days (change the water daily). For ethylene treatment, we reduced the amount of water to 1.5-1.7cm below the seeds.Three-day old seedlings were treated with ethylene (10 ppm) for 8 hours at 28Â°C in the dark. Seedling grown in the air was used as control. We isolated RNA from roots and shoots of wild-type and mutant.

2014-09-24 | E-GEOD-51153 | biostudies-arrayexpress

ENAP1 is involved in ethylene response

Project description:We report that ethylene regulate the ENAP1 binding. We perform a dual cross-linking procedure using the additional cross-linker ethylene glycol bis-succinimidylsuccinate (EGS; Thermo Scientific) along with formaldehyde according to (Pedmale et al. 2016) for Chip-sequencing (ChIPseq) of ENAP1, as well as ENAP1 ChIP- EIN2 reChIPseq (ChIP-reChIPseq), which were used chromatins isolated from 3-day old etiolated Col-0 seedlings treated with ethylene or air gas. Results show that ENAP1 is involved in the EIN2 dependent ethylene response.

2017-09-07 | GSE97288 | GEO

Ethylene and 1-MCP differentially regulate gene expression during onion (Allium cepa L.) sprout suppression

Project description:Onion is regarded as non-climacteric. In onion, ethylene can suppress sprouting however, the ethylene binding inhibitor, 1-MCP can also suppress sprout growth although it is unknown how ethylene and 1-MCP elicit the same response. In this study, onion bulbs were treated with 10 μL L-1 ethylene or 1 μL L-1 1-MCP individually or in combination for 24 h at 20°C before or after curing (six weeks) at 20 or 28°C then stored at 1°C. Following curing, a subset of these same onion bulbs was stored separately under continuous air or ethylene (10 μL L-1) at 1°C Six treatments were chosen for microarray analysis; four samples were taken before curing immediately after treatment with ethylene or 1-MCP or ethylene and 1-MCP in combination for 24 h at 20°C. The other two samples and two were taken at the end of storage following 6 weeks curing at 28°C andafter 29 weeks cold storage (1°C) in continuous air or continuous ethylene totalling 35 weeks storage. The four pre-curing samples used were untreated (control) or treated with EB, MB or EMB and the two samples after storage were control bulbs stored in continuous ethylene or air at 1°C. There were three biological replicates of each of the six treatments making 18 samples in total.

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Role of Macrophages in the Foreign Body Response to Poly(ethylene glycol) Hydrogels

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