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TMT-based quantitative proteomics analysis reveals the function of ester cyclase in the rice false smut fungus Ustilaginoidea virens

ABSTRACT: Rice false smut is a world-wide fungal disease caused by Ustilaginoidea virens. In this study, we found an ester cyclase, UvEC1, was significantly up-regulated during U. virens infection. UvEC1 contained a ketone cyclase Snoal-like polyketide cyclase domain and the functions of this important class of proteins in plant fungal pathogens remain unclear. Deletion of UvEC1 caused defects in vegetative growth and conidiation. UvEC1 is also required for response to hyperosmotic and oxidative stresses, and maintenance of cell wall integrity. Importantly, the ∆UvEC1 mutants exhibited reduced virulence. We performed an TMT-based quantitative proteomic analysis to identify differentially expressed proteins (DEPs) between ∆UvEC1-1 mutant and the wild type HWD-2. Proteomic data have revealed that UvEC1 have various effects on metabolism process, localization, catalytic activity, binding and spliceosome. Taken together, our findings suggest that UvEC1 is critical for development and virulence of U. virens.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ustilaginoidea Virens

TISSUE(S): Leaf

SUBMITTER: Pei zhangxin

LAB HEAD: LU Zheng

PROVIDER: PXD024701 | Pride | 2021-04-15

REPOSITORIES: Pride

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Proteomic analyses of lysine 2-hydroxyisobutyrylation in Fusarium graminearum in response to tebuconazole and carbendazim

Project description:Lysine 2-hydroxyisobutyrylation (Khib) is a newly discovered post-translational modification in some prokaryotic and eukaryotic organisms. Here, we carried out proteome-wide analysis of Khib in Fusarium graminearum, identifying the reshaping of lysine 2-hydroxyisobutyrylome by tebuconazole or carbendazim, using the most recently-developed high-resolution LC-MS/MS in combination with high-specific affinity enrichment. In total, 3035 quantifiable Khib sites on 937 proteins were identified. With the criteria of fold changes over 1.3, normalized with total proteins, 1083 Khib sites of 509 proteins were considered as significantly affected by tebuconazole treatment, while 344 Khib sites of 227 proteins were significantly affacted by carbendazim. Furthermore, bioinformatics analysis were conducted.

2022-04-04 | PXD022499 | Pride

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2020-12-02 | PXD019033 | Pride

Genotyping, generation and lysine 2-hydroxyisobutyrylation identification of the first human autosomal dominant osteopetrosis type II specific induced pluripotent stem cells

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