Proteomic analysis of exosomes isolated from S. Typhimurium-infected macrophages.
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ABSTRACT: Exosomes derived from S. Typhimurium-infected RAW264.7 macrophages (24 and 48 hpi) were isolated by differential ultracentrifugation. Equal protein samples in triplicates were subjected to protein extraction and separation by SDS-PAGE. Protein bands excised from each lane were reduced, alkylated, and digested with trypsin. Peptide pools were desalted by C18 columns and analyzed by liquid chromatography-Orbitrap Fusion tandem mass spectrometry
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Salmonella Typhimurium (strain Lt2 / Sgsc1412 / Atcc 700720) Mus Musculus (mouse)
TISSUE(S): Cell Culture, Macrophage
SUBMITTER: Mariola Edelmann
LAB HEAD: Mariola J Edelmann
PROVIDER: PXD024838 | Pride | 2021-04-02
REPOSITORIES: Pride
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