Proteomics

Dataset Information

0

Proteomic analysis of exosomes isolated from S. Typhimurium-infected macrophages.


ABSTRACT: Exosomes derived from S. Typhimurium-infected RAW264.7 macrophages (24 and 48 hpi) were isolated by differential ultracentrifugation. Equal protein samples in triplicates were subjected to protein extraction and separation by SDS-PAGE. Protein bands excised from each lane were reduced, alkylated, and digested with trypsin. Peptide pools were desalted by C18 columns and analyzed by liquid chromatography-Orbitrap Fusion tandem mass spectrometry

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Salmonella Typhimurium (strain Lt2 / Sgsc1412 / Atcc 700720) Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Mariola Edelmann  

LAB HEAD: Mariola J Edelmann

PROVIDER: PXD024838 | Pride | 2021-04-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
A1-p1.raw Raw
A1.raw Raw
A2-p1.raw Raw
A2.raw Raw
A3-p1.raw Raw
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