Proteomics

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Goat’s milk exosomes as natural nanoparticles in detection of inflammatory processes by optical imaging


ABSTRACT: Exosomes are cell-derived nanovesicles with a proven key role in intercellular signaling, inflammation processes and immune response. Due to their natural origin and liposome-like structure, these nanometric vesicles have emerged as novel platforms for therapy and diagnosis. This work presents goat’s milk exosomes as natural nanoparticles applied in the detection of inflammatory processes by optical imaging. Goat’s milk exosomes were isolated and fully characterized by their physicochemical properties, proteomics profile and biochemical impact in healthy mice. For the in vitro and in vivo assessment, these nanoparticles were covalently labeled with sulfo-Cyanine 5 and BODIPY-FL. In vitro studies confirmed the internalization of the nanoprobe as well as no cytotoxicity in macrophages populations (RAW 264.7). The capacity of our probe to localize inflammatory processes was in vivo assessed by optical imaging in a peritonitis mouse model confirmed. These results were also confirmed by the flow cytometry analysis of the peritonitis exudates, supporting the use of these natural nanoparticles in the detection of inflammatory processes.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Capra Hircus (goat)

TISSUE(S): Milk

DISEASE(S): Inflammation

SUBMITTER: Pilar Ximenez-Embun  

LAB HEAD: Javier Muñoz Peralta

PROVIDER: PXD025026 | Pride | 2022-02-21

REPOSITORIES: Pride

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Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.

Santos-Coquillat Ana A   González María Isabel MI   Clemente-Moragón Agustín A   González-Arjona Mario M   Albaladejo-García Virginia V   Peinado Héctor H   Muñoz Javier J   Ximénez Embún Pilar P   Ibañez Borja B   Oliver Eduardo E   Desco Manuel M   Salinas Beatriz B  

Small (Weinheim an der Bergstrasse, Germany) 20211202 6


Exosomes are cell-derived nanovesicles with a proven intercellular signaling role in inflammation processes and immune response. Due to their natural origin and liposome-like structure, these nanometer-scale vesicles have emerged as novel platforms for therapy and diagnosis. In this work, goat milk exosomes are isolated and fully characterized in terms of their physicochemical properties, proteomics, and biochemical profile in healthy mice, and used to detect inflammatory processes by optical im  ...[more]

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