Proteomics

Dataset Information

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Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice


ABSTRACT: Accurate analysis of tissue-specific secretory proteins in blood plasma can provide valuable information regarding therapeutic target development but current approaches are mostly limited to ex vivo proteomic analysis. Here, we developed an in situ secretory protein labeling technology using endoplasmic reticulum-anchored TurboID (iSLET) and validated its utility in vivo by the successful identification of liver-specific secretory proteins in mouse plasma. iSLET is a promising new strategy that will facilitate the discovery of tissue-specific secretory proteins mediating interorgan communication in normal physiology and disease.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Blood Plasma, Cell Culture, Early Embryonic Cell

SUBMITTER: Jong-Seo Kim  

LAB HEAD: Jong-Seo Kim

PROVIDER: PXD025141 | Pride | 2021-08-06

REPOSITORIES: Pride

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