Dataset Information

Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum

ABSTRACT: Diplonema papillatum represents a group of highly diverse and abundant marine protists with still unknown lifestyle and ecological functions. Based on alterations of the transcriptomic, proteomic and metabolomic profiles obtained from cells grown under different conditions we designed a metabolic map of its cellular bioenergetic pathways. Comparative analysis in the nutrient-rich and -poor media and in the absence and presence of oxygen revealed a capacity for major metabolic reprograming. D. papillatum is equipped with fundamental metabolic routes such as glycolysis, gluconeogenesis, TCA cycle, pentose phosphate pathway, respiratory complexes, β-oxidation and synthesis of fatty acid. While gluconeogenesis uniquely dominates over glycolysis, TCA cycle represents a combination of standard and unusual enzymes. The presence of typical anaerobic enzymes such as pyruvate:NADP+ oxidoreductase, fumarate reductase, opine dehydrogenase, enoyl-coenzyme A reductase, and lactate dehydrogenase reflects the ability to survive in low-oxygen environments. The metabolism quickly reacts to restricted carbon source, revealing unusual flexibility of diplonemids, also reflected in cell morphology and motility, which is in good correlation with their extreme ecological valence.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Diplonema Papillatum

SUBMITTER: Peter Barath

LAB HEAD: Peter Barath

PROVIDER: PXD025411 | Pride | 2021-11-15

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	201228_Diplo_-O2_1_01.raw	Raw
	201228_Diplo_-O2_1_02.raw	Raw
	201228_Diplo_-O2_1_04.raw	Raw
	201228_Diplo_-O2_2_01.raw	Raw
	201228_Diplo_-O2_2_02.raw	Raw

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Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum.

Škodová-Sveráková Ingrid I Záhonová Kristína K Juricová Valéria V Danchenko Maksym M Moos Martin M Baráth Peter P Prokopchuk Galina G Butenko Anzhelika A Lukáčová Veronika V Kohútová Lenka L Bučková Barbora B Horák Aleš A Faktorová Drahomíra D Horváth Anton A Šimek Petr P Lukeš Julius J

BMC biology 20211124 1

<h4>Background</h4>The phylum Euglenozoa is a group of flagellated protists comprising the diplonemids, euglenids, symbiontids, and kinetoplastids. The diplonemids are highly abundant and speciose, and recent tools have rendered the best studied representative, Diplonema papillatum, genetically tractable. However, despite the high diversity of diplonemids, their lifestyles, ecological functions, and even primary energy source are mostly unknown.<h4>Results</h4>We designed a metabolic map of D. p ...[more]

PMID: 34819072

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