Proteomics

Dataset Information

0

Proteomics of Yucatan minipig cartilages


ABSTRACT: Ten cartilages, including hyaline, fibrous, and elastic cartilages, were analyzed via LC-MS/MS bottom-up proteomics analysis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

SUBMITTER: Clinton Yu  

LAB HEAD: Dr. Kyriacos Athanasiou

PROVIDER: PXD025482 | Pride | 2022-06-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BB_OrbitrapLUMOS_20201212_01.raw Raw
BB_OrbitrapLUMOS_20201212_03.raw Raw
BB_OrbitrapLUMOS_20201212_05.raw Raw
BB_OrbitrapLUMOS_20201212_07.raw Raw
BB_OrbitrapLUMOS_20201212_09.raw Raw
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Publications

Methodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages.

Bielajew Benjamin J BJ   Hu Jerry C JC   Athanasiou Kyriacos A KA  

Cartilage 20211126 2_suppl


<h4>Introduction</h4>This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig.<h4>Methods</h4>For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. T  ...[more]

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