Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)
SUBMITTER: Clinton Yu
LAB HEAD: Dr. Kyriacos Athanasiou
PROVIDER: PXD025482 | Pride | 2022-06-09
REPOSITORIES: Pride
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Bielajew Benjamin J BJ Hu Jerry C JC Athanasiou Kyriacos A KA
Cartilage 20211126 2_suppl
<h4>Introduction</h4>This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig.<h4>Methods</h4>For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. T ...[more]