Proteomics

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Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis


ABSTRACT: Some carboxydotrophs like Rhodospirillum rubrum are able to grow with CO as their sole source of energy using a Carbone monoxide dehydrogenase (CODH) and an Energy conserving hydrogenase (ECH) to perform anaerobically the so called water-gas shift reaction (WGSR) (CO + H2O-> CO2 + H2). Several studies have focused at the biochemical and biophysical level on this enzymatic system and a few OMICS studies on CO metabolism. Knowing that CO is toxic in particular due to its binding to heme iron atoms, and is even considered as a potential antibacterial agent, we decided to use a proteomic approach in order to analyze R. rubrum adaptation in term of metabolism and management of the toxic effect. In particular, this study allowed highlighting a set of proteins likely implicated in ECH maturation, and important perturbations in term of cofactor biosynthesis, especially metallic cofactors. This shows that even this CO tolerant microorganism cannot avoid completely CO toxic effects associated with its interaction with metallic ions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rhodospirillum Rubrum (strain Atcc 11170 / Ncib 8255)

TISSUE(S): Cell Culture

SUBMITTER: Hélène Diemer  

LAB HEAD: Cianferani Sarah

PROVIDER: PXD025489 | Pride | 2024-09-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
QE29999HD.mgf Mgf
QE29999HD.mzid.gz Mzid
QE29999HD.raw Raw
QE30001HD.mgf Mgf
QE30001HD.mzid.gz Mzid
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