Dataset Information

Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis

ABSTRACT: Some carboxydotrophs like Rhodospirillum rubrum are able to grow with CO as their sole source of energy using a Carbone monoxide dehydrogenase (CODH) and an Energy conserving hydrogenase (ECH) to perform anaerobically the so called water-gas shift reaction (WGSR) (CO + H2O-> CO2 + H2). Several studies have focused at the biochemical and biophysical level on this enzymatic system and a few OMICS studies on CO metabolism. Knowing that CO is toxic in particular due to its binding to heme iron atoms, and is even considered as a potential antibacterial agent, we decided to use a proteomic approach in order to analyze R. rubrum adaptation in term of metabolism and management of the toxic effect. In particular, this study allowed highlighting a set of proteins likely implicated in ECH maturation, and important perturbations in term of cofactor biosynthesis, especially metallic cofactors. This shows that even this CO tolerant microorganism cannot avoid completely CO toxic effects associated with its interaction with metallic ions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rhodospirillum Rubrum (strain Atcc 11170 / Ncib 8255)

TISSUE(S): Cell Culture

SUBMITTER: Hélène Diemer

LAB HEAD: Cianferani Sarah

PROVIDER: PXD025489 | Pride | 2024-09-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 30

Similar Datasets

Project description:Plastics are one of the most preoccupying emerging pollutants. Macroplastics released in the environment degrade into microplastics and nanoplastics. Because of their small size, these micro and nano plastic particles can enter the food chain and, in addition to their ecotoxicological effects, contaminate humans with still poorly known biological effects. Plastics being particulate pollutants, they are handled in the human body by scavenger cells such as macrophages, which are important players in the immune system. Because of all the potential problems, it is advocated to replace fossil fuel-based plastics by bio-based and bio-degradable plastics, among which poly-hydroxyalkanoates are the most promising. However, the effects of these on mammalian cells are even less known than those of fossil fuel-based plastics. We therefore designed a study aiming at investigating the effects of polylactic acid (PLA) nanoparticles on macrophages. Indeed, being a plastic, PLA is known to fragment and liberate micro and nanoparticles, exactly as conventional plastics. These particles will be internalized by macrophages and may induce functional consequences on these cells. Proteomics showed important adaptive changes of the proteome in response to exposure to PLA, and several important pathways such as mitochondrion, lysosomes or endoplasmic reticulum were highlighted by the proteomic analysis. However, validation experiments showed that most of these changes were homeostatic and allowed the cells to keep these functions unaltered. When the inflammatory response was examined, no major increase in the secretion of tumor necrosis factor or interleukin 6 was observed. However, the secretion of these cytokines in response to lipopolysaccharide was altered after exposure to PLA. The production of interleukin 6 was decreased, while the production of tumor necrosis factor, showing a complex alteration of cellular responses after exposure to PLA nanoparticles. In conclusion, these results provide a better understanding of the responses of macrophages to exposure to the biodegradable PLA nanoparticles.

			Action	DRS
	QE29999HD.mgf	Mgf
	QE29999HD.mzid.gz	Mzid
	QE29999HD.raw	Raw
	QE30001HD.mgf	Mgf
	QE30001HD.mzid.gz	Mzid

Dataset Information

Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets