Proteomics

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SARS-Cov2 coronavirus spike protein-induced apoptosis, inflammatory and oxidative stress responses in THP-1-Like-Macrophages: potential role of angiotensin converting enzyme inhibitor (perindopril)


ABSTRACT: A purified spike (S) glycoprotein for SARS-COV-2 coronavirus was used to studying its effects on THP-1 macrophages, PBMCs and HUVEC cells. The S protein mediates SARS-CoV-2 entry into cells through binding to angiotensin converting enzyme 2 (ACE2) receptors. We measured viability, intracellular cytokines release, oxidative stress, pro-inflammatory markers and THP-1-like macrophage polarization. We identified an increase in apoptosis, ROS generation, MCP-1 and intracellular calcium expression in THP-1 macrophages. Furthermore, stimulation with the S protein polarizes THP-1 macrophages towards pro-inflammatory futures with an increase in TNFα and MHC-II M1-like phenotype markers. Treatment of cells with an ACE inhibitor, perindopril at 100nM reduced apoptosis, ROS and MHC-II expression. We further analyzed the sensitivity of HUVEC cells after exposure to a conditioned media (CM) of THP-1 macrophages stimulated with S protein. CM induced endothelial cell apoptosis and MCP-1 expression. Treatment with perindopril reduced these effects. However, direct stimulation of HUVEC cells with S protein slightly increased HIF1α and MCP-1 expression which was significantly exaggerated by ACE inhibitor treatment. S protein stimulation induced ROS generation and changed mitogenic responses of PBMCs through upregulation of TNFα and IL-17 cytokine expressions. These effects were blunted by perindopril (100nM) treatment. Proteomic analysis of S protein stimulated THP-1 macrophages with or without perindopril (100nM) uncovered more than 400 differentially regulated proteins. Our results provide a mechanistic analysis suggesting that blood and vascular component could be activated directly through S protein systemically present in circulation and that activation of local renin angiotensin system might be partially involved in this process.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human) Severe Acute Respiratory Syndrome Coronavirus 2

TISSUE(S): Monocytic Leukemia Cell Line, Macrophage

SUBMITTER: Bandar Alghanem  

LAB HEAD: MOHAMED BOUDJELAL

PROVIDER: PXD025516 | Pride | 2021-11-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MS5600-101320-GB-11.group Other
MS5600-101320-GB-11.wiff Wiff
MS5600-101320-GB-11.wiff.scan Wiff
MS5600-101320-GB-12.group Other
MS5600-101320-GB-12.wiff Wiff
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