Proteomics

Dataset Information

0

Screening for Ku70 phosphoS155-dependent proximal proteins

ABSTRACT: To identify Ku70 phosphoS155-specific proximal protein interactors, we used the proximity-dependent biotin identification (BioID2) technique with wild-type Ku70, Ku70 S155A, and Ku70 S155D in the doxycycline-inducible T-REx 293 cell line and compared the different datasets.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Sanna Abbasi  

LAB HEAD: Caroline Schild-Poulter

PROVIDER: PXD025581 | Pride | 2023-05-10

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
peptides_1_1_0_Green_191118182223.mzid.gz Mzid
peptides_1_1_0_NLSTREX_1.mzid.gz Mzid
peptides_1_1_0_NLSTREX_2.mzid.gz Mzid
peptides_1_1_0_NLSTREX_3.mzid.gz Mzid
peptides_1_1_0_Red.mzid.gz Mzid
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Publications

Analysis of Ku70 S155 Phospho-Specific BioID2 Interactome Identifies Ku Association with TRIP12 in Response to DNA Damage.

Abbasi Sanna S   Bayat Laila L   Schild-Poulter Caroline C  

International journal of molecular sciences 20230411 8

The Ku heterodimer, composed of subunits Ku70 and Ku80, is known for its essential role in repairing double-stranded DNA breaks via non-homologous end joining (NHEJ). We previously identified Ku70 S155 as a novel phosphorylation site within the von Willebrand A-like (vWA) domain of Ku70 and documented an altered DNA damage response in cells expressing a Ku70 S155D phosphomimetic mutant. Here, we conducted proximity-dependent biotin identification (BioID2) screening using wild-type Ku70, Ku70 S15  ...[more]

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