Dataset Information

Quantitative proteomic screen identifies Annexin A2 as a host target for Salmonella pathogenicity island-2 effectors SopD2 and PipB2

ABSTRACT: In this work we use stable isotope labeling of amino acids in cell culture (SILAC) and a S. Typhimurium mutant that secretes increased amounts of effectors to identify cognate effector binding partners during infection. Using this method, we identified the host protein annexin A2 as a binding partner for both SopD2 and PipB2 and were able to confirm its binding to SopD2 by reciprocal pull down. This indicates that SopD2 and PipB2 likely both interact with annexin A2 through protein-protein interactions to establish the S. Typhimurium intracellular replicativeniche. This demonstrates the value of studying effector interactions using proteomic techniques and natural effector delivery during infection rather than transfection.

INSTRUMENT(S): Bruker Daltonics instrument model

ORGANISM(S): Homo Sapiens (human) Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. 5579 Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. A130

SUBMITTER: Jenny Moon

LAB HEAD: Leonard J. Foster

PROVIDER: PXD025582 | Pride | 2022-02-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	KK_1-1_190818_A1_01_4252.d.1.dar	Other
	KK_1-2_190818_A2_01_4253.d.1.dar	Other
	KK_1-3_190818_A3_01_4255.d.1.dar	Other
	KK_1-4_190818_A4_01_4256.d.1.dar	Other
	KK_1-5_190818_A5_01_4257.d.1.dar	Other

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Publications

Quantitative proteomic screen identifies annexin A2 as a host target for Salmonella pathogenicity island-2 effectors SopD2 and PipB2.

Knuff-Janzen Katelyn K Serapio-Palacios Antonio A McCoy James J Krekhno Zakhar Z Moon Kyung-Mee KM Deng Wanyin W Foster Leonard J LJ Finlay B Brett BB

Scientific reports 20211208 1

Intracellular pathogens need to establish an intracellular replicative niche to promote survival and replication within the hostile environment inside the host cell. Salmonella enterica serovar Typhimurium (S. Typhimurium) initiates formation of the unique Salmonella-containing vacuole and an extensive network of Salmonella-induced tubules in order to survive and thrive within host cells. At least six effectors secreted by the type III secretion system encoded within Salmonella pathogenicity isl ...[more]

PMID: 34880286

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