Dataset Information

Human colorectal cancer LC-MS/MS

ABSTRACT: The global crotonylome of human colorectal cancer and paired paracancerous tissues using quantitative proteomics.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

SUBMITTER: Jiayi Hou

LAB HEAD: Jiayi Hou

PROVIDER: PXD025709 | Pride | 2023-10-24

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	F9316LPCr_N_2_Slot1-42_1_1350.d.zip	Other
	F9316LPCr_N_3_Slot1-44_1_1354.d.zip	Other
	F9316LPCr_N_4_Slot1-46_1_1358.d.zip	Other
	F9316LPCr_N_6_Slot1-48_1_1362.d.zip	Other
	F9316LPCr_T_2_Slot1-41_1_1348.d.zip	Other

Items per page:

1 - 5 of 9

Publications

Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer.

Hou Jia-Yi JY Li Ning N Wang Jie J Gao Li-Juan LJ Chang Jia-Song JS Cao Ji-Min JM

Epigenetics & chromatin 20230926 1

<h4>Background</h4>Blood-based tests have public appeal in screening cancers due to their minimally invasive nature, ability to integrate with other routine blood tests, and high compliance. This study aimed to investigate whether certain epigenetic modulation of peripheral blood mononuclear cells (PBMCs) could be a biomarker of colorectal cancer (CRC).<h4>Results</h4>Western blotting of histones in the PBMCs from 40 colorectal cancer patients and 40 healthy controls was performed to identify th ...[more]

PMID: 37749610

Similar Datasets

Project description:N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteomes in microalgal systems. Here, N-glycoproteome of the model diatom Phaeodactylum tricornutum was unveiled. Totally, 893 different N-glycosylated sites from 649 proteins were identified from P. tricornutum. The new synthesized N-glycans were principally transferred to asparagine residues within the conserved N-X-S/T (where X is a residue other than proline) sequence of nascent polypeptide chains. Functional annotation of N-glycosylated proteins showed that 70% N-glycoproteins were involved in vesicular transport and posttranslational modification, protein turnover, chaperones, indicating the N-glycosylation was important for these functions. Of the all identified N-glycoproteins 45.3% were predicted to localize on chloroplast, which implied the widespread regulatory role of N-glycosylation in chloroplast. Furthermore, the enrichment results of N-glycoproteins indicated that compared to ‘Cellular component’ and ‘Biological process’ categories proteins related with the ‘Molecular function’ category were more prone to be N-glycosylated. And it was speculated that N-glycosylation played a vital regulatory role in catalytic activity of enzymes and metabolism processes of many small molecules. 47% of all enriched proteins were related with metabolic pathways. The functional annotation and enrichment of N-glycoproteins suggested that N-glycoproteins participated in a variety of important metabolic pathways and perform different functions in P. tricornutum. 12 proteins involved in the ER quality control mechanism and ER- associated degradation pathway were identified as N-glycosylated proteins, indicating that the N-glycosylated modification was important for their functions in the protein N-glycosylation pathway. Additionally, some interacted glycoproteins were classified from this study, which provided valuable information for studying the functions of these glycoproteins. In the study, the identification of N-glycosylation on nascent proteins expands our understanding of this PTM at a proteomics scale and may facilitate the elucidation of the precise function of proteins in this model diatom.

Project description:Background: Cylindrocladium parasiticum Crous, Wingfield & Alfenas, the causal agent of Cylindrocladium black rot (CBR) of peanut (Arachis hypogaea L.), has leaded to economic losses in China. Learning about how peanut responds to C. parasiticum infection will be conducive to designing strategies for CBR control. However, the response of peanut plant to C. parasiticum is poorly understood. Results: In this study, two contrasting peanut cultivars, T09 (C. parasiticum-resistant) and P562 (C. parasiticum-susceptible) were used for comparative analysis of protein profiles in the root segment of peanut plants in responses to C. parasiticum infection. Proteomic profiling identified 1647 and 391 differentially expressed proteins (DEPs) in A. hypogaea L. P562 and A. hypogaea L. T09, respectively, compared to controls. A total of 350 and 1095 DEPs were identified between A. hypogaea L. P562 and A. hypogaea L. T09 before and after 9 dpi, respectively. Functional categorization by GO annotation showed that C. parasiticum-responsive proteins were mainly involved in catalytic activity and binding. The results of KEGG pathway analysis indicated both resistant and susceptible peanut cultivars can regulate gene expression in the phenylpropanoid pathway, terpenoid backbone biosynthesis, SA, and JA pathways to induce defensive genes and protein expression which enhances plant defence capacity. However, the MAPK signal pathway was more pronounced in resistant peanut cultivar T09. We also observed an increase of CYP73A100 involved in phenylpropanoid biosynthesis and flavonoid biosynthesis pathways in the susceptible peanut ecotype P562, while decrease in the resistant peanut ecotype T09, after 9 dpi. Additionally, there was a marked activation of brassinosteroid biosynthesis in the resistant T09, which indicated a possible involvement of activation of plant immune response in the resistant responses of peanut to C. parasiticum. Conclusions: This study provides some insights into the molecular networks involved on cellular and physiological responses to C. parasiticum infestation.