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Proteomics of cardiac stromal cells and dermal fibroblasts secretomes

ABSTRACT: The proteomics characterization of the secretomes as well as the evaluation of their potential to induce cell and tissue responses are widely used strategies. Here are presented the mass spectrometry data of the resident cardiac stromal cells (CRSC) and dermal fibroblasts secretomes, which enables the comparison of their compositions.

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast Of Dermis, Stromal Cell

SUBMITTER: Michel Batista

LAB HEAD: Alessandra Melo de Aguiar

PROVIDER: PXD026451 | Pride | 2022-02-07

REPOSITORIES: Pride

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			Action	DRS
	20141205_nCM1_1.RAW	Raw
	20141205_nCM1_2.RAW	Raw
	20141205_nCM1_3.RAW	Raw
	20141205_vCM3_1.RAW	Raw
	20141205_vCM3_2.RAW	Raw

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Publications

Secretome from resident cardiac stromal cells stimulates proliferation, cardiomyogenesis and angiogenesis of progenitor cells.

Reus Thamile Luciane TL Robert Anny Waloski AW Da Costa Marise Brenner Affonso MB de Aguiar Alessandra Melo AM Stimamiglio Marco Augusto MA

International journal of cardiology 20160630

In the heart, tissue-derived signals play a central role on recruiting/activating stem cell sources to induce cardiac lineage specification for maintenance of tissue homeostasis and repair. Cardiac resident stromal cells (CRSCs) may play a pivotal role in cardiac repair throughout their secretome. Here, we performed the characterization of CRSCs and their secretome by analyzing the composition of their culture-derived extracellular matrix (ECM) and conditioned medium (CM) and by investigating th ...[more]

PMID: 27404713

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Project description:Exogenous signals from drug-stressed cancer cells promote acquisition of a more aggressive phenotype of neighboring tumor cells. Recently, we examined how cancer cell secretome changes in response to chemotherapy. We showed that tumor therapy-induced secretomes significantly increased resistance of cancer cells to subsequent cisplatin treatment but we did not observed the same phenomenon on normal fibroblasts. To elucidate how therapy-induced secretomes affect the transcriptomic profiles of recipient tumor cells, we incubated SKOV3 cells for 3 days with secretomes from control or dying cancer cells. The same conditions were used for normal fibroblasts. Enrichment analysis of differentially expressed genes in SKOV3 cells incubated with therapy-induced secretomes identified prominent up-regulation of genes involved in coordination of mitotic processes, G2/M transition checkpoints and DNA repair. These findings demonstrate that under stress condition cancer but not normal cells can secrete signaling molecules into the exracellular space and contribute to the emergence of tumor chemoresistance during short time period.

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Matrisome of neonatal mouse dermal fibroblasts

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RNA-seq of mesenchymal stromal cells cultured in the presence of breast cancer cell secretomes

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2019-05-28 | E-MTAB-6998 | biostudies-arrayexpress

Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: Not as effective as cells

Project description:Although the paracrine effects of mesenchymal stem/stromal cells (MSCs) have been recognized as crucial mediators of their regenerative effects on tissue repair, the potential of MSC secretomes as effective substitutes for cellular therapies remains underexplored. In this study, we compared MSCs from the human dermis (DSCs) and adipose tissue (ASCs) with their secretomes regarding their efficacy for skin wound healing using a translationally-relevant murine model. Proteomic analysis revealed that while there was a substantial overlap in protein composition between DSC and ASC secretomes, specific proteins associated with wound healing and angiogenesis were differentially expressed. Despite a similar angiogenic potential in vivo, DSC- and ASC-secretomes were found to be less effective than cells in accelerating wound closure and promoting tissue remodeling. Overall, secretome-treated groups showed intermediary results between cells and control (empty scaffold) treated groups. These findings highlight that although secretomes possess therapeutic potential, their efficacy might be limited compared to cellular therapies. This study contributes to the growing understanding of MSC secretomes, emphasizes the need for further protocol optimization, and offers insights into their potential applications in regenerative medicine.

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Intrapericardial administration of secretomes from endometrial-derived mesenchymal stromal cells: effects on immune-related genes in a porcine model of myocardial infarction

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Response of human corneal epithelial cells to the secretomes of wild-type Serratia marcescens and an eepR mutant strain

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Porcine dermal extracellular matrix LC-MSMS

Project description:Hitherto, microenvironments provided by dermal analogues could not avoid frequent split-thickness skin grafts (STSGs) failure and scar fibrosis. We reported a novel method assembling 3D-printed dermal analogues (PDAs) with porcine dermal extracellular matrix(dECM)'s recapitulated the latter’s compositional and topological features. These optimized PADs reduced skin wounds contraction and improved cosmetic upshots proving superior to STSGs and commercially available dermal templates. Once grafted onto wounds beds, optimized PDAs evoked a type-2-like immune response, activated type-2 macrophages (M2-Mφ), upregulated anti-inflammatory genes like Wnt11, Fgf16 and ATF3, downregulated profibrotic genes like Il13r⍺2, Itg⍺11, and IL1β, and hindered fibrosis. Thus, PDAs' beneficial effects resulted from preserved dermis-specific ECM cues (by mass spectrometry analysis we identified more than 200 unique protein species inside our dECM powder) and well-balanced physicochemical properties, which collaboratively modulated crucial endogenous signaling pathways.

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Single-cell RNA-seq of of human dermal fibroblasts (supplementary - different stimulation conditions of dsRNA)

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