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Matrisome of neonatal mouse dermal fibroblasts

ABSTRACT: The goal of this project is to compare the protein composition of the extracellular matrices (ECMs) deposited in vitro by wild type, heterozygous, or SPAG17 KO neonatal mouse dermal fibroblasts.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Fibroblast, Skin Fibroblast

SUBMITTER: George Chlipala

LAB HEAD: Alexandra Naba

PROVIDER: PXD019869 | Pride | 2022-09-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	142_2.dat	Other
	142_2.mgf	Mgf
	142_2.mzid.gz	Mzid
	142_2.raw	Raw
	167-1.dat	Other

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1 - 5 of 60

Publications

Reduced SPAG17 Expression in Systemic Sclerosis Triggers Myofibroblast Transition and Drives Fibrosis.

Sapao Paulene P Roberson Elisha D O EDO Shi Bo B Assassi Shervin S Skaug Brian B Lee Fred F Naba Alexandra A Perez White Bethany E BE Córdova-Fletes Carlos C Tsou Pei-Suen PS Sawalha Amr H AH Gudjonsson Johann E JE Ma Feiyang F Verma Priyanka P Bhattacharyya Dibyendu D Carns Mary M Strauss Jerome F JF Sicard Delphine D Tschumperlin Daniel J DJ Champer Melissa I MI Campagnola Paul J PJ Teves Maria E ME Varga John J

The Journal of investigative dermatology 20220915 2

Systemic sclerosis (SSc) is a clinically heterogeneous fibrotic disease with no effective treatment. Myofibroblasts are responsible for unresolving synchronous skin and internal organ fibrosis in SSc, but the drivers of sustained myofibroblast activation remain poorly understood. Using unbiased transcriptome analysis of skin biopsies, we identified the downregulation of SPAG17 in multiple independent cohorts of patients with SSc, and by orthogonal approaches, we observed a significant negative c ...[more]

PMID: 36116512

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Extracellular matrix derived from oral mesenchymal stem cells

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Dental Pulp Stem Cells LC-MS/MS analysis

Project description:This study focuses on the impact of ECM (extra-cellular matrix) over cell processes such as proliferation, differentiation or mineralization which is more specifically related to our dental pulp cells. We cultured these dental pulp stem cells (DPSC) in mineralization growth or normal growth conditions. After 21 days, cells were incubated in decellularized solution until no intact cells are seen. After ECM was washed, we studied the mineralization associated to these two different ECM. Matrisome proteins were identified through proteomics. DPSC matrisome is composed of 225 individual different proteins. We classified them according to different categories, the 3 core matrisome categories: glycoproteins, collagens, proteoglycans and the 3 matrisome associated proteins categories: the regulators, affiliated and secreted. When comparing the proteins in the N-ECM and OM-ECM, most of the core matrisome proteins are downregulated in OM conditions, except 3 glycoproteins, as well as regulators and secreted factors. However, annexins were found to be upregulated in OM condition. Cell adhesion, tensile strength and growth factor binding are over-represented in NM ECM. The collagen group and glycoproteins were higher in N-ECM than OM-ECM Thereafter, gingival stem cells (GSC), the less inherit osteogenic potency cells, were seeded on these N-ECM and OM-ECM. When GSCs were seeded on DPSC-derived ECM, the OM-ECM dramatically promoted mineral deposition compared with N-ECM. We hypothesize that annexins could participate in the osteogenic inductive properties. ECM plays a pivotal role in many physiological processes, it regulates cells behavior and can orient cell differentiation. Dental pulp and oral mucosa share embryological origins but differ in their reactions to insults. Dental pulp can mineralize while oral mucosa heals ad integrum. We hypothesize that ECM participate in these characteristics.

2021-09-23 | PXD018951 | Pride

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