Dataset Information

Spatially restricted proteomics reveals dynamic changes in the caveolin-1 interactome in response to increased membrane tension

ABSTRACT: Here, we used APEX2 proximity labelling technique coupled with mass spectrometry to define Cveolin-1 interactome at steady state and after hypo-osmotic shock. We created a stable cell line expressing Caveolin1-APEX2-EGFP in human retinal pigmented epithelial cells (RPE-1). Hypo osmotic shock was performed accordingly to Sinha et al, 2011. Briefly, cells were incubated in 30mOsm medium for 5 minutes (Hypo condition). To analyse the ability to recover, after the osmotic shock cells were allowed to recover in isotonic medium for 30 minutes (REC condition). Cells grown in iso-tonic medium were used as normal non treated condition (NT). RPE-1 cells expressing APEX2-EGFP in the cytoplasm (NES-APEX2-EGFP) was used as control to exclude highly abundant cytosolic proteins.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Retinal Pigment Epithelial Cell

SUBMITTER: Rossana Girardello

LAB HEAD: Gunnar Dittmar

PROVIDER: PXD026464 | Pride | 2024-11-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Hs_CAV1clf_A2E_Uniprot9606C_20200106.fasta	Fasta
	Oxidation__M_Sites.txt	Txt
	Qplus_00028504_MQQC_HS_NES-NTA.raw	Raw
	Qplus_00028506_MQQC_HS_NES-NTC.raw	Raw
	Qplus_00028507_MQQC_HS_NES-NTD.raw	Raw

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Publications

Time-resolved proximity proteomics uncovers a membrane tension-sensitive caveolin-1 interactome at the rear of migrating cells.

Martin Eleanor E Girardello Rossana R Dittmar Gunnar G Ludwig Alexander A

eLife 20240924

Caveolae are small membrane pits with fundamental roles in mechanotransduction. Several studies have shown that caveolae flatten out in response to increased membrane tension, thereby acting as a mechanosensitive membrane reservoir that buffers acute mechanical stress. Caveolae have also been implicated in the control of RhoA/ROCK-mediated actomyosin contractility at the rear of migrating cells. However, how membrane tension controls the organisation of caveolae and their role in mechanotransduc ...[more]

PMID: 39315773

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