Dataset Information

Human-derived PBMCs were stimulated by different groups to kill tumor cells.After 48 hours, PBMCs were taken out, and the enrichment of activated proteins of relevant lymphocytes and the regulation of pathways were examined by proteomics.

ABSTRACT: In this study, proteomics was used to explore the activation of lymphocytes and related pathways in PBMCs of different stimulation groups. In our study, proteomics analysis showed that the immune cells in PBMCs activated by stimulation group exhibit improved functions related to white blood cell activation, proliferation and cytotoxicity during biological processes. At the same time, KEGG pathway analysis showed that the signaling pathways’ key proteins for leukocyte transendothelial migration, Th1/Th2 cell differentiation, JAK-STAT (involved in many important biological processes such as cell proliferation, differentiation, apoptosis and immune regulation), DNA replication and Th17 cell differentiation were up-regulated; whereas the key proteins of necroptosis pathways in immune cells were down-regulated.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Peripheral Blood Mononuclear Cell, Cell Culture

DISEASE(S): Melanoma

SUBMITTER: Jing Jin

LAB HEAD: Binlei Liu

PROVIDER: PXD026739 | Pride | 2023-07-20

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 16

Publications

Bispecific Antibody Expressed by an Oncolytic Herpes Simplex Virus Type 2 Can Transform Heterologous T Cells Into Uniform Tumor Killer Cells.

Jin Jing J Wang Runyang R Yang Junhan J Hu Han H Wang Di D Cai Linkang L Fang Zhizheng Z Dong Shuang S Hu Sheng S Wang Yang Y Liu Binlei B

Human gene therapy 20220601 11-12

BsAb (bispecific antibody)-armed oncolytic viruses (OVs) are effective in regulating tumor microenvironment. However, oHSV2 (oncolytic herpes simplex virus type 2) expressing immune checkpoints targeting BsAb molecules are not reported. Here, we generated oHSV2-armed PD-L1/CD3 BsAb and established pharmacodynamic evaluation models, which suggested that our oHSV2-BsAb molecules have an improved oncolytic potency <i>in vitro</i> and <i>in vivo.</i> The oHSV2 viruses armed with BsAb molecules targe ...[more]

PMID: 35272497

Similar Datasets

Project description:The overriding objective of this application is to seek funding for the development of a crossectional profile of immune phenotype of over 600 normal subjects between the ages of 50 and 90. As a consequence, we will infer changes in immune phenotype of normal subjects as they age. These immune phenotypic data, as well as standard laboratory tests and evaluations of questionnaires, will be used to generate a large and comprehensive database of demographic and biological information. The proposal utilizes the strengths of the Stanford Human Immune Monitoring Core (HIMC) and the unique expertise in basic immunology, immune monitoring, the development of patient registries, biostatistics and bioinformatics at Stanford. The dataset will comprise a crossectional analysis of the local San Francisco Peninsula general population between the ages of 50 and 90 (representing equal gender and representative ethnic population, and equal distribution by decade of life). The registry will contain demographic data, race/ethnicity, prescribed medications, over the counter medications, vitamins, alternative therapies, physical function questionnaire, alternative contact person, and HIPPA release. Fasting blood will be obtained for immune phenotyping. The immune profile will contain the results of both conventional and novel immune profiling assays to develop the normative immune profile of aging (using PBMC subset analysis, cytokines, and activation induced signaling of PBMCs for phosphoepitope and gene expression analyses). Data from these analyses will be useful in identifying biomarkers of the normal immune profile associated with aging as well as correlation with phenotypic aspects of aging such as sarcopenia and disability The immune profile (as well as normal blood chemistries and demographic data) of these subjects will be made available to serve as the basis for future longitudinal study of change in the immune profile over time in association with the development of co-morbidities associated with aging. in the PBMC gene expression, 120 samples were analyzed (no replicates), containing patients and controls.

			Action	DRS
	WD022TQ_1.raw	Raw
	WD022TQ_10.raw	Raw
	WD022TQ_11.raw	Raw
	WD022TQ_12.raw	Raw
	WD022TQ_13.raw	Raw